Expression and function of the nonclassical estrogen receptor, GPR30, in human cartilage and chondrocytes

Madalena Ribeiro; Catia Sousa; Ana T. RufinoFerno JudasAlexrina F. Mendes

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Expression and function of the nonclassical estrogen receptor, GPR30, in human cartilage and chondrocytes

机译：的表达和功能模人类软骨和雌激素受体GPR30在软骨细胞

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Estrogen hormones are important for cartilage homeostasis, but nothing is known regarding the expression and role of the membrane G protein-coupled estrogen receptor (GPER), G protein-coupled receptor 30 (GPR30), in adult articular chondrocytes. Using immunohistochemistry of cartilage sections, quantitative real-time polymerase chain reaction and Western blot of chondrocyte extracts, we found that these cells express GPR30. Nonetheless, the pattern of bands detected by two distinct antibodies does not overlap, suggesting that the proteins detected represent partially degraded forms of the receptor. Treatment with GPR30 agonists did not induce Akt or ERK1/2 phosphorylation, two known GPR30-activated signaling pathways, suggesting that GPR30 is not functional in human chondrocytes. Therefore, the protective anti-osteoarthritic role of estrogen hormones in cartilage homeostasis is likely independent of GPR30. This study was performed using human cartilage collected from the distal femoral condyles of multiorgan donors at the Bone and Tissue Bank of the University and Hospital Center of Coimbra.

机译：雌激素荷尔蒙对软骨很重要体内平衡,但没有什么是已知的有关表达和作用膜的Gprotein-coupled雌激素受体(gp), G30 (protein-coupled受体GPR30),在成人关节软骨细胞。免疫组织化学的软骨部分,定量实时聚合酶链反应和免疫印迹的软骨细胞提取物,我们发现这些细胞表达GPR30。然而,乐队的模式检测到两个不同的抗体不重叠,建议蛋白质的发现代表部分退化形式的受体。GPR30受体激动剂不能产生一种蛋白激酶或ERK1/2磷酸化,两个已知GPR30-activated信号通路,这表明GPR30不是人类软骨细胞功能。保护anti-osteoarthritic雌激素的作用激素在软骨内稳态是可能的GPR30的独立。从远端使用人类软骨收集股骨髁部multiorgan捐助者的骨头和组织的大学和医院Coimbra的中心。

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《Journal of cellular physiology.》 |2020年第11期|8486-8494|共9页
作者
Madalena Ribeiro; Catia Sousa; Ana T. RufinoFerno JudasAlexrina F. Mendes;
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Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal;

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正文语种英语
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G protein-coupled estrogen receptor 1; Chondrocyte; CartilageReal-Time Polymerase Chain ReactionTissue BanksEstrogen ReceptorsEstradiol;

机译：G protein-coupled雌激素受体1;软骨细胞;CartilageReal-Time聚合酶链式ReactionTissue BanksEstrogen ReceptorsEstradiol;

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Expression and function of the nonclassical estrogen receptor, GPR30, in human cartilage and chondrocytes

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