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首页> 外文期刊>Journal of cellular physiology. >Isolation and characterization of breast cancer stem cell-like phenotype by Oct4 promoter-mediated activity
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Isolation and characterization of breast cancer stem cell-like phenotype by Oct4 promoter-mediated activity

机译：乳腺癌的隔离和表征由Oct4干细胞的表型promoter-mediated活动

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Cancer stem cells (CSCs) are a small subset of cancer cells responsible for self-renewal activity, drug resistance, and tumor recurrence. CSCs have been derived from diverse tumors and cell lines. The expression of sternness markers has been identified in CSCs. Oct4 is a well-established transcription factor expressed in stem cells and CSCs. In this study, we isolated and characterized breast CSC-like cells from murine MC4-L2 cells by Oct4 promoter-mediated activity. The MC4-L2 cells were electroporated by a plasmid expressing puromycin resistance (Puro~R) gene from the Oct4 promoter and then selected by puromycin. The isolated cells were named as the MC4-L2~(puro) cells and characterized for CSCs properties. Immunostaining indicated CD44~(high) and CD24~(high) phenotype for the MC4-L2 and MC4-L2~(puro) cells. The enhanced expression of stem cell markers was detected in the puromycin-selected cells compared with the parental cells. Moreover, the isolated cells only grew up in sphere-formed shape in low attachment plates. Serial dilution transplantation in syngeneic mouse models showed increased tumorigenicity of the MC4-L2~(puro) cells, as they induced new tumors when injected into the mammary fat pad as few as 104 cells. In conclusion, we designed a novel genetic construct, which allows the isolation of Oct4-positive cells in a cancer population by a simple selection step in a puromycin-containing medium. Transfection of this construct into the MC4-L2 cells resulted in growing a subpopulation of cells having tumor-initiating cell characteristics. To the best of our knowledge, this is the first report on the isolation of CSC-like cells from the mouse breast cancer MC4-L2 cells.
机译：癌症干细胞(二者)是一个小的子集肿瘤细胞自我更新负责活动、耐药性以及肿瘤复发。二者都来自不同的肿瘤和细胞系。在二者已被确认。的转录因子表达在干细胞和二者。孤立和乳房CSC-like细胞特点从小鼠MC4-L2 Oct4细胞promoter-mediated活动。electroporated由质粒表达嘌呤霉素电阻(普罗~ R)从Oct4基因启动子然后由嘌呤霉素。细胞被命名为MC4-L2 ~(嘌呤霉素)细胞为二者属性特征。表示CD44 ~(高)和CD24 ~(高)表型MC4-L2和MC4-L2 ~(嘌呤霉素)细胞。加强干细胞标记物的表达发现在puromycin-selected细胞相比与父母的细胞。细胞在低只在sphere-formed长大的形状附件盘子。移植在同系的小鼠模型致瘤性的增加MC4-L2 ~(嘌呤霉素)细胞,诱导新肿瘤注射到乳腺脂肪垫只有104个细胞。结论,我们设计了一个新的基因构造,它允许隔离的人口由Oct4-positive细胞癌症简单的puromycin-containing选择一步媒介。MC4-L2细胞导致一个分组人口增长细胞在肿瘤起源细胞特征。这是第一次报告的隔离CSC-like从小鼠乳腺癌细胞MC4-L2细胞。

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