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首页> 外文期刊>Journal of cellular physiology. >Autophagy protects PC12 cells against deoxynivalenol toxicity via the Class III PI3K/beclin 1/Bcl-2 pathway
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Autophagy protects PC12 cells against deoxynivalenol toxicity via the Class III PI3K/beclin 1/Bcl-2 pathway

机译：自噬保护PC12细胞通过第三类deoxynivalenol毒性PI3K / beclin 1 / bcl - 2通路

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摘要

Deoxynivalenol (DON) is a major mycotoxin from the trichothecene family of mycotoxins produced by Fusarium fungi. It can cause a variety of adverse effects on human and farm animal health. Here, we determined the effect of DON on the Class III phosphatidylinositol 3-kinase (PIK3C3)/beclin 1/B cell lymphoma-2 (Bcl-2) pathway in PC12 cells and the relationship between autophagy and apoptosis. The effects of DON were evaluated based on the apoptosis ratio; the typical indicators of autophagy, including cellular morphology, acridine orange- and monodansylcadaverine-labeled vacuoles, green fluorescent protein-microtubule associated protein 1 light chain 3 (LC3) localization, and LC3 immunofluorescence; and the expression of key autophagy-related genes and proteins, that is, PIK3C3, beclin 1, Bcl-2, LC3, and p62. The relationship between autophagy and apoptosis was analyzed by western blot analysis and flow cytometry. DON-induced PC12 cell morphological changes and autophagy significantly. PIK3C3, beclin 1, and LC3 increased in tandem with the DON concentration used; Bcl-2 and p62 expression decreased as DON concentrations increased. Moreover, the PIK3C3/beclin l/Bcl-2 signaling pathway played a role in DON-induced autophagy. Our findings suggest that DON can induce autophagy by activating the PIK3C3/beclin l/Bcl-2 signaling pathway and that autophagy may play a positive role in reducing DON-induced apoptosis.
机译：Deoxynivalenol(不)是一个主要的真菌毒素单端孢霉烯族产生的真菌毒素镰刀菌素真菌。对人类和家畜健康的影响。确定并在第三类的效果磷脂酰肌醇3-kinase (PIK3C3) / beclin 1 / B细胞lymphoma-2 (bcl - 2)在PC12细胞和途径自噬与凋亡之间的关系。的影响并进行评估的基础上细胞凋亡率;自噬,包括细胞形态,吖啶橙- monodansylcadaverine-labeled绿色荧光protein-microtubule液泡相关蛋白1轻链3 (LC3)本地化和LC3免疫荧光;autophagy-related基因和表达的关键蛋白质,PIK3C3 beclin 1 bcl - 2, LC3,和p62。细胞凋亡分析了免疫印迹分析和流式细胞术。形态变化和自噬显著。增加与浓度使用;浓度增加。PIK3C3 / beclin l / bcl - 2信号通路发挥了在DON-induced自噬作用。建议也可以诱导自噬激活PIK3C3 / beclin l / bcl - 2信号途径,自噬可能发挥积极在减少DON-induced细胞凋亡的作用。

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