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首页> 外文期刊>Journal of cellular physiology. >MFN2 silencing promotes neural differentiation of embryonic stem cells via the Akt signaling pathway
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MFN2 silencing promotes neural differentiation of embryonic stem cells via the Akt signaling pathway

机译：沉默中进行MFN2促进神经分化的作用胚胎干细胞通过一种蛋白激酶信号通路

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摘要

Mitofusin 2 (MFN2) is a regulatory protein participating in mitochondria dynamics, cell proliferation, death, differentiation, and so on. This study aims at revealing the functional role of MFN2 in the pluripotency maintenance and primitive differetiation of embryonic stem cell (ESCs). A dox inducible silencing and routine overexpressing approach was used to downregulate and upregulate MFN2 expression, respectively. We have compared the morphology, cell proliferation, and expression level of pluripotent genes in various groups. We also used directed differentiation methods to test the differentiation capacity of various groups. The Akt signaling pathway was explored by the western blot assay. MFN2 upregulation in ESCs exhibited a typical cell morphology and similar cell proliferation, but decreased pluripotent gene markers. In addition, MFN2 overexpression inhibited ESCs differentiation into the mesendoderm, while MFN2 silencing ESCs exhibited a normal cell morphology, slower cell proliferation and elevated pluripotency markers. For differentiation, MFN2 silencing ESCs exhibited enhanced three germs' differentiation ability. Moreover, the protein levels of phosphorylated Akt308 and Akt473 decreased in MFN2 silenced ESCs, and recovered in the neural differentiation process. When treated with the Akt inhibitor, the neural differentiation capacity of the MFN2 silenced ESCs can reverse to a normal level. Taken together, the data indicated that the appropriate level of MFN2 expression is essential for pluripotency and differentiation capacity in ESCs. The increased neural differentiation ability by MFN2 silencing is strongly related to the Akt signaling pathway.
机译：进行MFN2 Mitofusin 2()是一个监管的蛋白质参与线粒体动力学,细胞扩散、死亡、分化,等等。本研究旨在揭示的功能作用进行MFN2的多能性和维护原始微分的胚胎干细胞(的ESCs)。overexpressing方法被用来表达下调分别进行MFN2和上调表达。比较形态学、细胞增殖,多能性基因的表达水平各种各样的团体。分化的方法来测试各种组织的分化能力。一种蛋白激酶信号通路被西方探索污点分析。典型的细胞形态和类似的细胞扩散,但减少多能性基因标记。ESCs抑制分化成的进行MFN2 mesendoderm,沉默的ESCs展出正常细胞形态、细胞慢核扩散和高架多能性标记。ESCs分化,进行MFN2沉默表现出增强的三个细菌的分化能力。磷酸化Akt308 Akt473下降ESCs进行MFN2沉默,恢复神经分化的过程。一种蛋白激酶抑制剂,神经分化ESCs的沉默中进行MFN2可以扭转的能力一个正常的水平。表明,适当的进行MFN2水平表达多能性和至关重要ESCs分化能力。神经分化能力进行MFN2沉默是一种蛋白激酶信号通路密切相关。

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