...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular physiology. >Transcriptional profiling of genes in tongue epithelial tissue from immature and adult rats by the RNA-Seq technique
【24h】

Transcriptional profiling of genes in tongue epithelial tissue from immature and adult rats by the RNA-Seq technique

机译：基因的转录剖析的舌头上皮组织的成熟和成年大鼠RNA-Seq技术

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Children are more sensitive than adults to bitterness and thus dislike bitter tastes more than adults do. However, why children are more sensitive to bitterness has never been revealed. To elucidate the effects of age on taste perception, a double-bottle preference test was first performed with immature and adult rats. Then, RNA-Seq analysis was performed on tongues obtained from rats of the same ages as those in the double-bottle test. The immature rats exhibited a lower consumption rate of bitter solution than the adult rats. Bioinformatics analysis yielded 1,347 differentially expressed genes (DEGs) between male adult rats (MARs, 80 days old) and male immature rats (MIRs, 20 days old) and 380 DEGs between female adult rats (FARs, 80 days old) and female immature rats (FIRs, 20 days old). These DEGs were mainly associated with growth, development, differentiation, and extracellular processes, among other mechanisms. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the DEGs were enriched for bitter taste transduction. Specifically, the Gnb3 and TRPM5 genes were downregulated in FARs compared with FIRs and in MARs compared with MIRs, and the protein expression of TRPM5 was significantly downregulated in MARs compared with MIRs. The data presented herein suggest that transcriptional regulation of taste-associated signal transduction occurs differently in tongue epithelial tissue of rats at different ages, although additional analyses are needed to confirm this conclusion.
机译：儿童比成人更敏感苦,因此不喜欢苦涩的味道比成人做的事。对痛苦敏感从未透露。阐明年龄对味道的影响知觉,double-bottle偏好测试第一个表现不成熟和成年老鼠。然后,RNA-Seq分析在舌头上从大鼠获得相同的年龄的double-bottle测试。表现出较低的消费率的苦比成年鼠的解决方案。分析了1347个差异表达基因(度)之间的男性成年老鼠(火星,80天)和“男性未成熟大鼠(大鹏展翅,20天老女成年老鼠之间)和380度(80天的法尔斯)和女性不成熟的老鼠(冷杉,20天)。与生长、发育,分化和细胞外的过程,其他机制。百科全书的基因和基因组(KEGG)通路分析,富集度苦味转导。相比,法尔斯基因表达下调冷杉,在火星与大鹏展翅,TRPM5明显的蛋白表达表达下调相比,火星有大鹏。本文提供的数据表明转录调节taste-associated信号转导发生不同的舌头大鼠在不同年龄段的上皮组织,虽然需要更多的分析证实这一结论。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号