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>Long noncoding RNA LINC00629 restrains the progression of gastric cancer by upregulatingAQP4 through competitively binding to miR-196b-5p
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Long noncoding RNA LINC00629 restrains the progression of gastric cancer by upregulatingAQP4 through competitively binding to miR-196b-5p
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机译：长非编码RNA LINC00629抑制由upregulatingAQP4胃癌的进展通过竞争性结合mir - 196 b - 5 - p
Gastric cancer continues to be a common cancer in the world with high incidence and mortality. Accumulating evidence has implicated long noncoding RNAs (IncRNAs) in gastric cancer progression. Here, this study identified the potential role of a novel IncRNA, LINC00629 in gastric cancer and to elucidate the underlying mechanism. Initially, microarray-based gene expression profiling of gastric cancer was employed to identify differentially expressed genes. Next, the expression of LINC00629, microRNA-196b-5p (miR-196b-5p) and aquaporin 4 (AQP4) in clinical gastric cancer tissues was determined and the cell line presenting with the lowest LINC00629 expression was selected. The interaction among LINC00629, miR-196b-5p, and AQP4 was identified. Expression of LINC00629, miR-196b-5p, and AQP4 in gastric cancer cells were altered and then biological behaviors of gastric cancer cells were assessed by 5-ethynyl-2'-deoxyuridine and Transwell assays. Tumor formation in vivo was evaluated in nude mice. In gastric cancer, expression of LINC00629 and AQP4 was downregulated, and expression of miR-196b-5p was upregulated. Proliferation, invasion, and migration of gastric cancer cells were reduced after overexpression of LINC00629. LINC00629 competitively bound to miR-196b-5p, while AQP4 was a target of miR-196b-5p. Either downregulating miR-196b-5p or upregulating AQP4 could restrain the development of gastric cancer in vitro. LINC00629 overexpression repressed the growth of transplanted tumors in vivo. Taken together, LINC00629 competitively bound to miR-196b-5p to upregulate AQP4 expression, thereby inhibiting gastric cancer progression. Therefore, understanding of this mechanism may help to improve gastric cancer treatment.
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机译：胃癌仍是一种常见的癌症世界上高发病率和死亡率。越来越多的证据牵连非编码rna在胃癌(IncRNAs)进展。一本小说的潜在作用IncRNA, LINC00629胃癌和阐明底层机制。胃癌的表达分析用来识别差异表达基因。微rna - 196 b - 5 - p (mir - 196 b - 5 - p)和水通道蛋白4(AQP4)在临床胃癌组织决定的细胞系呈现最低LINC00629表达式被选中。LINC00629之间互动,mir - 196 b - 5 - p和AQP4被确认。mir - 196 b - 5 - p,和AQP4在胃癌细胞生物的行为被改变了,然后呢胃癌细胞被评估5-ethynyl-2脱氧尿苷和Transwell化验。肿瘤形成体内是裸体的评估老鼠。和AQP4表达下调和表达mir - 196 b - 5 - p是调节。入侵,胃癌细胞的迁移减少过度LINC00629后。LINC00629竞争力必将mir - 196 b - 5 - p,AQP4的目标时mir - 196 b - 5 - p。表达下调mir - 196 b - 5 - p或者移植AQP4能抑制胃癌的发展吗体外。体内移植肿瘤的生长。绑定到一起,LINC00629竞争力mir - 196 b - 5 - p上调AQP4表达,从而抑制胃癌进展。因此,这种机制的理解有助于改善胃癌治疗。
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