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首页> 外文期刊>Journal of cellular physiology. >PCBP2 promotes the development of glioma by regulating FHL3/TGF-β/Smad signaling pathway
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PCBP2 promotes the development of glioma by regulating FHL3/TGF-β/Smad signaling pathway

机译：PCBP2促进神经胶质瘤的发展调节FHL3 / TGF -β/ Smad信号通路

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The purpose of this study was to investigate the role of Poly (C)-binding protein 2 (PCBP2) and the related signaling pathway in glioma progression. Quantitative realtime polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were performed to measure PCBP2 messenger RNA and protein expression in glioma tissues or cells. Cell transfection was completed using Lipofectamine 2000. 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Transwell assay and flow cytometry assay were used to explore the effects of PCBP2 expression on biological behaviors of glioma cells. Western blot assay was used for the detection of pathway related proteins. Expression of PCBP2 in glioma tissues and cells were higher than that in paracancerous tissues and normal cells (both p < .01). Moreover, the elevated expression of PCBP2 was significantly correlated with tumor size (p = .001) and WHO stage (p = .010). Knockdown of PCBP2 could suppress proliferation, migration and invasion of glioma cells and promote apoptosis. Besides, the expression of transforming growth factor-β (TGF-β) pathway related proteins TGF-β1, p-Smad2 and p-Smad7 were decreased following the downregulation of PCBP2. PCBP2 also inhibited FHL3 expression by binding to FHL3-3'UTR. The inhibition of FHL3 could reverse the antitumor action caused by PCBP2 silencing. In vivo assay, PCBP2 was also found to inhibit the tumor growth of glioma. PCBP2 activates TGF-β/Smad signaling pathway by inhibiting FHL3 expression, thus promoting the development and progression of glioma.
机译：本研究的目的是调查保利(C)的角色绑定蛋白2 (PCBP2)和神经胶质瘤的相关信号通路进展。(存在)和免疫组织化学链反应(包含IHC)进行测量PCBP2信使RNA和蛋白表达在神经胶质瘤组织中细胞。Lipofectamine 2000。5-diphenyltetrazolium 5-Dimethylthiazol-2-yl) 2溴化分析,Transwell化验和流式细胞术分析被用来探索PCBP2的影响表情神经胶质瘤的生物学行为细胞。检测通路相关蛋白。神经胶质瘤组织和细胞中的PCBP2更高比paracancerous组织和正常细胞(p < . 01)。表达PCBP2显著相关与肿瘤大小(p =措施)和世卫组织阶段(p =.010)。神经胶质瘤增殖、迁移和入侵细胞,促进细胞凋亡。转化生长因子-β的表情(TGF -β)通路相关蛋白TGF -β1,p-Smad2和p-Smad7后下降downregulation PCBP2。通过绑定FHL3-3'UTR FHL3表达式。抑制FHL3可以逆转抗肿瘤行动由PCBP2沉默造成的。PCBP2也发现抑制肿瘤的生长神经胶质瘤。通路通过抑制FHL3表达,因此促进的开发和发展神经胶质瘤。

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