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首页> 外文期刊>Journal of cellular physiology. >Focal adhesion kinase promotes BMP2-induced osteogenic differentiation of human urinary stem cells via AMPK and Wnt signaling pathways
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Focal adhesion kinase promotes BMP2-induced osteogenic differentiation of human urinary stem cells via AMPK and Wnt signaling pathways

机译：粘着斑激酶促进BMP2-induced人类尿干细胞的成骨分化细胞通过AMPK和Wnt信号通路

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摘要

Human urine-derived stem cells (hUSCs) serve as favorable candidates for bone transplants due to their efficient proliferative and multipotent differentiation abilities, as well as the capacity to secrete a variety of vasoactive agents to facilitate tissue engineering. The present study aimed to explore the role of focal adhesion kinase (FAK) in bone morphogenetic protein 2 (BMP2)-induced osteogenic differentiation of hUSCs and to investigate the underlying mechanism. The degree of osteogenic differentiation and the correlated signals, following BMP2 overexpression and siRNA-mediated silencing of FAK, were determined in vitro. Moreover, hUSCs induced bone formation in a rat model with cranial defects, in vivo. Our findings revealed that alkaline phosphatase production, calcium deposits, osteocalcin and osteopontin expression, and bone formation were upregulated in vitro and in vivo following BMP2-induced osteogenic differentiation, and AMPK and Wnt signaling pathway activation by FAK could effectively regulate BMP2-enhanced osteogenic differentiation of hUSCs. Taken together, these findings indicated that FAK could mediate BMP2-enhanced osteogenic differentiation of hUSCs through activating adenosine 5'-monophosphate-activated protein kinase and Wnt signaling pathways.
机译：人类urine-derived干细胞(hUSCs)担任良好的骨移植由于候选人他们有效的增殖和多功能分化能力,以及分泌多种血管活性的能力代理商促进组织工程。本研究旨在探讨焦点的作用黏附激酶(FAK)在骨形成全身的成骨蛋白2 (BMP2)分化hUSCs和调查底层机制。分化和相关信号,后BMP2过度和siRNA-mediated沉默的FAK,测定体外。此外,hUSCs诱导骨形成在一只老鼠与颅骨缺损模型,体内。表明,碱性磷酸酶生产,钙沉积,骨钙蛋白和骨桥蛋白表情,骨形成和调节在体外和体内BMP2-induced成骨分化,AMPK和Wnt由FAK信号通路的激活能有效地调节BMP2-enhanced成骨的hUSCs分化。研究结果表明,FAK可以调解hUSCs BMP2-enhanced成骨分化通过激活腺苷酸5 ' -monophosphate-activated蛋白激酶和Wnt信号通路。

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