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首页> 外文期刊>Journal of cellular physiology. >Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smadl/5 signaling pathway
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Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smadl/5 signaling pathway

机译：阿司匹林促进tenogenic分化肌腱干细胞和促进病变通过调节愈合GDF7 / Smadl / 5信号通路

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摘要

Tendinopathy is a common musculoskeletal system disorder in sports medicine, but regeneration ability of injury tendon is limited. Tendon stem cells (TSCs) have shown the definitive treatment evidence for tendinopathy and tendon injuries due to their tenogenesis capacity. Aspirin, as the representative of nonsteroidal anti-inflammatory drugs for its anti-inflammatory and analgestic actions, has been commonly used in treating tendinopathy in clinical, but the effect of aspirin on tenogenesis of TSCs is unclear. We hypothesized that aspirin could promote injury tendon healing through inducing TSCs tenogenesis. The aim of the present study is to make clear the effect of aspirin on TSC tenogenesis and tendon healing in tendinopathy, and thus provide new treatment evidence and strategy of aspirin for clinical practice. First, TSCs were treated with aspirin under tenogenic medium for 3, 7, and 14 days. Sirius Red staining was performed to observe the TSC differentiation. Furthermore, RNA sequencing was utilized to screen out different genes between the induction group and aspirin treatment group. Then, we identified the filtrated molecules and compared their effect on tenogenesis and related signaling pathway. At last, we constructed the tendinopathy model and compared biomechanical changes after aspirin intake. From the results, we found that aspirin promoted tenogenesis of TSCs. RNA sequencing showed that growth differentiation factor 6 (GDF6), GDF7, and GDF11 were upregulated in induction medium with the aspirin group compared with the induction medium group. GDF7 increased tenogenesis and activated Smadl/5 signaling. In addition, aspirin increased the expression of TNC, TNMD, and Scx and biomechanical properties of the injured tendon. In conclusion, aspirin promoted TSC tenogenesis and tendinopathy healing through GDF7/Smadl/5 signaling, and this provided new treatment evidence of aspirin for tendinopathy and tendon injuries.
机译：病变是一种常见的肌肉骨骼系统在运动医学疾病,但再生损伤肌腱的能力是有限的。细胞(tsc)显示的治疗腱子病变衰退的证据和肌腱损伤tenogenesis能力。代表非甾体类抗炎药物的抗炎和analgestic行动,是常用的治疗在临床病变,但效果的阿司匹林对tsc tenogenesis尚不清楚。假设阿司匹林可以促进损伤肌腱愈合通过诱导tsc tenogenesis。本研究的目的是明确的阿司匹林对TSC tenogenesis和肌腱在病变的愈合,从而提供新的阿司匹林的治疗证据和策略临床实践。阿司匹林在tenogenic介质3、7和14天。观察TSC分化。测序是用来屏蔽不同基因之间的诱导组和阿司匹林治疗组。相比有机分子和他们的影响tenogenesis和相关信号通路。最后,我们病变模型和构造比较阿司匹林后生物力学变化的摄入量。促进了tsc的tenogenesis。表明,生长分化因子6(GDF6) GDF7, GDF11调节感应介质与阿司匹林组比较与感应介质组。tenogenesis和激活Smadl / 5信号。另外,阿斯匹林的表达增加过渡委员会,TNMD Scx和生物力学特性的肌腱受伤。促进了TSC tenogenesis和病变的愈合通过GDF7 / Smadl / 5信号,这提供新的证据表明阿司匹林治疗病变和肌腱损伤。

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