Kp-10 promotes bovine mammary epithelial cell proliferation by activating GPR54 and its downstream signaling pathways

Yanwei Li; Yu Cao; Jiaxin WangShoupeng FuJi ChengLijun MaQing ZhangWenjin GuoXingchi KanJuxiong Liu

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Kp-10 promotes bovine mammary epithelial cell proliferation by activating GPR54 and its downstream signaling pathways

机译：Kp-10促进牛乳腺上皮细胞通过激活GPR54及其增殖下游信号通路

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It has been reported that the proliferation and apoptosis of mammary epithelial cells affect milk production. Therefore, ensuring adequate mammary epithelial cells is expected to enhance milk production. This study is devoted to studying the effects of kisspeptin-10 (Kp-10), a peptide hormone composed of 10 amino acids, on bovine mammary epithelial cell (bMEC) proliferation and exploring the underlying mechanism of its action. bMECs were treated with various concentrations of Kp-10 (1, 10, 100, and 1,000 nM), and 100 nM Kp-10 promoted the proliferation of the bMECs. Kp-10 promoted the cell cycle transition from Gl to the S and G2 phases, increased the protein levels of Cyclin Dl and Cyclin D3, and reduced the expression levels of the p21 gene. This study also showed that inhibition of G protein-coupled receptor 54 (GPR54), AKT, mTOR, and ERK1/2 reduced the proliferation of the bMECs that had been induced by Kp-10. In addition, Kp-10 decreased the complexes formed by Rb and E2F1 and increased the expression levels of the E2F1 target genes. These results indicate that Kp-10 promotes bMEC proliferation by activating GPR54 and its downstream signaling pathways.

机译：据报道,核扩散和乳腺上皮细胞的细胞凋亡影响牛奶生产。上皮细胞有望提高牛奶生产。肽的影响kisspeptin-10 (Kp-10)10个氨基酸组成的激素,在牛乳腺上皮细胞(bMEC)增殖和探索其作用的潜在机制。bMECs治疗各种浓度的Kp-10(1、10、100和1000海里),和100海里Kp-10提升bMECs的扩散。从Gl Kp-10促进了细胞周期转变年代和G2阶段,增加蛋白质细胞周期蛋白Dl水平和细胞周期素D3,减少p21的表达水平。还显示,抑制G protein-coupledreceptor 54 (GPR54)行为,mTOR, and ERK1/2减少的bMECs扩散被Kp-10诱导。减少Rb和E2F1形成的复合物E2F1的表达水平增加目标基因。通过激活GPR54促进bMEC扩散及其下游信号通路。

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《Journal of cellular physiology.》 |2020年第5期|4481-4493|共13页
作者
Yanwei Li; Yu Cao; Jiaxin WangShoupeng FuJi ChengLijun MaQing ZhangWenjin GuoXingchi KanJuxiong Liu;
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Department of Theoretic Veterinary Medicine, College of Veterinary Medicine, Jilin University;

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正文语种英语
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EIF4EBP1 gene; Signal Pathways; KISS1R protein, humanCyclin D3G2 PhaseMilk productionCell Proliferationcyclin Dl;

机译：EIF4EBP1基因;信号途径;KISS1R蛋白质,humanCyclin D3G2 PhaseMilk productionCellProliferationcyclin Dl;

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Kp-10 promotes bovine mammary epithelial cell proliferation by activating GPR54 and its downstream signaling pathways

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