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首页> 外文期刊>Journal of cellular physiology. >Circular RNA circFNDC3B protects renal carcinoma by miR-99a downregulation
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Circular RNA circFNDC3B protects renal carcinoma by miR-99a downregulation

机译：环状RNA circFNDC3B保护肾癌通过。差别mir - 99 a对这些

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摘要

Various circular RNAs (circRNAs) have been reported to involve in carcinoma. This study explored the role and mechanism of circRNA circFNDC3B (circFNDC3B) in renal carcinoma (RC). The detection indicators in this paper were viability, colony, and migration, which respectively investigated by Cell Counting Kit-8, colony formation, and migration assay. Reverse transcriptase quantitative polymerase chain reaction tested and cell transfection changed circFNDC3B and miR-99a expression. Moreover, western blot tested relate-proteins of proliferation, migration, and cell pathways were examined by western blot. circFNDC3B was upregulated at RC tissues. circFNDC3B enhanced cell viability, colony and migration, and miR-99a mimic played reverse impacts. Furthermore, circFNDC3B negatively regulated miR-99aand circFNDC3B restrained the janus kinase I/signal transducer and activator of transcription 3 (JAK1/STAT3) and extracellular signal-regulated kinase (ERK) kinase (MEK)/ERK pathways by miR-99a downregulation. Overexpression of circFNDC3B enhanced cell viability, colony formation and migration by miR-99a downregulation via JAK1/STAT3 and MEK/ERK pathways.
机译：各种圆形rna (circRNAs)据报道,参与癌。探讨了circRNA的作用和机制circFNDC3B (circFNDC3B)在肾癌(RC)。本文的检测指标可行性、殖民地和迁移分别调查Kit-8细胞计数,集落形成和迁移试验。转录酶定量聚合酶链反应测试和细胞转染改变circFNDC3B, mir - 99 a的表情。免疫印迹检测relate-proteins增殖、迁移和细胞通路通过免疫印迹检测。调节在RC组织。细胞生存能力、殖民地和迁移和mir - 99 a模拟了反向影响。circFNDC3B消极监管mir - 99 - 20circFNDC3B克制janus激酶I /信号传感器和转录激活3(JAK1 / STAT3)和细胞外signal-regulated激酶(ERK)激酶(MEK / ERK途径mir - 99 adownregulation。增强细胞活力,形成和殖民地通过差别mir - 99 a对这些移民JAK1 / STAT3和MEK / ERK途径。

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