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首页> 外文期刊>Journal of cellular physiology. >circ-CEP85L suppresses the proliferation and invasion of gastric cancer by regulating NFKBIA expression via miR-942-5p
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circ-CEP85L suppresses the proliferation and invasion of gastric cancer by regulating NFKBIA expression via miR-942-5p

机译：circ-CEP85L增殖和抑制调节NFKBIA入侵胃癌表达式通过mir - 942 - 5 - p

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摘要

The expression pattern and role of circular RNAs (circRNAs) in the pathogenesis of gastric cancer (GC) and their underlying mechanisms remain unresolved. In this study, we identified differentially expressed circRNAs by a circRNA microarray and verified the results by quantitative reverse transcription-polymerase chain reaction using 117 clinical samples. Cell Counting Kit-8, wound healing, Transwell, and tumorsphere formation assays were conducted to assess the effects of circ-CEP85L on cell proliferation and invasion in vitro. Mouse intraperitoneal injection models were used to assess the functions of circ-CEP85L in vivo. Luciferase reporter assays, fluorescence in situ hybridization, and rescue experiments were performed to elucidate the underlying mechanism of circ-CEP85L. We found that circ-CEP85L, which has not been studied in GC, was significantly downregulated in GC tissues and that decreased circ-CEP85L expression correlated significantly with a worse prognosis. The knockdown of circ-CEP85L promoted the proliferation and invasion of GC cells, which was reversed by overexpression of circ-CEP85L. Furthermore, inhibition of circ-CEP85L promoted tumor growth in vivo. Mechanistically, circ-CEP85L was confirmed to be a direct target of miR-942-5p. In addition, rescue experiments indicated that circ-CEP85L is able to inhibit the proliferation and invasion of GC cells by sponging miR-942-5p. Finally, western blot assays verified that the down regulation of miR-942-5p efficiently reversed the inhibition of NFKBIA induced by circ-CEP85L overexpression. Therefore, we conclude that circ-CEP85L promotes NFKBIA expression by acting as a sponge of miR-942-5p; thus, inhibiting GC proliferation and invasion. circ-CEP85L is a potential target in the treatment of GC.
机译：圆形rna的表达模式和作用(circRNAs)在胃癌的发病机理(GC)和他们的潜在机制仍然存在没有解决。差异表达circRNAs circRNA微阵列和验证结果定量反向transcription-polymerase使用117份临床样本的连锁反应。计算Kit-8,伤口愈合、Transwell和tumorsphere形成进行了分析评估circ-CEP85L对细胞的影响体外增殖和入侵。腹腔内注射模型被用来评估circ-CEP85L体内的功能。荧光素酶记者分析,荧光原位杂交,救援实验阐明了潜在的机制circ-CEP85L。没有研究GC,明显吗在GC组织表达下调,降低了circ-CEP85L表达显著相关预后差。circ-CEP85L扩散和推广入侵GC细胞,逆转的circ-CEP85L超表达。抑制circ-CEP85L促进了肿瘤的生长体内。确认的直接目标mir - 942 - 5 - p。此外,救援实验表明circ-CEP85L能够抑制增殖和入侵GC细胞通过骗取mir - 942 - 5 - p。最后,免疫印迹分析验证监管的有效mir - 942 - 5 - p逆转NFKBIA诱导的抑制circ-CEP85L超表达。得出结论:circ-CEP85L促进NFKBIA表达式的海绵作为mir - 942 - 5 - p;因此,抑制GC扩散和入侵。circ-CEP85L是一个潜在的目标治疗GC。

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