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首页> 外文期刊>Journal of cellular physiology. >Testin regulates the blood-testis barrier via disturbing occludin/ZO-1 association and actin organization
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Testin regulates the blood-testis barrier via disturbing occludin/ZO-1 association and actin organization

机译：Testin调节blood-testis障碍通过令人不安的occludin / ZO-1协会和肌动蛋白组织

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摘要

The blood-testis barrier (BTB) separates the seminiferous epithelium into the apical and basal compartments. The BTB has to operate timely and accurately to ensure the correct migration of germ cells, meanwhile maintaining the immunological barrier. Testin was first characterized from primary Sertoli cells, it is a secretory protein and a sensitive biomarker to monitor junctions between Sertoli and germ cells. Till now, the functions of testin on BTB dynamics and the involving mechanisms are unknown. Herein, testin acts as a regulatory protein on BTB integrity. In vitro testin knockdown by RNAi caused significant damage to the Sertoli cell barrier with no apparent changes in the protein levels of several major tight junction (TJ), adhesion junction, and gap junction proteins. Also, testin RNAi caused the diffusion of two TJ structural proteins, occludin and ZO-1, diffusing away from the Sertoli cell surface into the cytoplasm. Association and colocalization between ZO-1 and occludin were decreased after testin RNAi, examined by Co-IP and coimmunofluorescent staining, respectively. Furthermore, testin RNAi induced a dramatic disruption on the arrangement of actin filament bundles and a reduced F-actin/G-actin ratio. The actin regulatory protein ARP3 appeared at the Sertoli cell interface after testin RNAi without its protein level change, whereas overexpressing testin in Sertoli cells showed no effect on TJ barrier integrity. The above findings suggest that besides as a monitor for Sertoli-germ cell junction integrity, testin is also an essential molecule to maintain Sertoli-Sertoli junctions.
机译：blood-testis障碍(BTB)分离精上皮细胞顶端和基底隔间。准确,以确保正确的迁移生殖细胞,同时维护免疫屏障。从主要支持细胞的特点,这是一个分泌蛋白和敏感的生物标志物监控滋养和生殖细胞之间的连接。直到现在,功能testin BTB动力学和涉及的机制是未知的。testin充当监管BTB的蛋白质的完整性。塞尔托利氏细胞造成了重大损失障碍没有明显变化的蛋白质水平的几个主要紧密连接(TJ),粘附连接、缝隙连接蛋白。此外,testin RNAi两TJ的扩散引起的结构蛋白,occludin ZO-1,扩散塞尔托利氏细胞表面进入细胞质。ZO-1和occludin testin后下降RNAi,检查Co-IP coimmunofluorescent分别染色。诱导一个戏剧性的安排中断肌动蛋白丝束和减少f -肌动蛋白/ G-actin比率。塞尔托利氏细胞蛋白质ARP3出现界面后testin RNAi没有蛋白质水平变化,而overexpressing testin塞尔托利氏细胞没有影响TJ障碍的完整性。除了作为Sertoli-germ监控单元结的完整性,testin也是必不可少的分子保持Sertoli-Sertoli连接。

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