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首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Estimation of fundamental kinetic parameters of polyhydroxybutyrate fermentation process of azohydromonas australica using statistical approach of media optimization
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Estimation of fundamental kinetic parameters of polyhydroxybutyrate fermentation process of azohydromonas australica using statistical approach of media optimization

机译：介质最优化统计方法估算澳大利亚偶氮氢单胞菌多羟基丁酸酯发酵过程基本动力学参数

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摘要

Polyhydroxybutyrate or PHB is a biodegradable and biocompatible thermoplastic with many interesting applications in medicine, food packaging, and tissue engineering materials. The present study deals with the enhanced production of PHB by Azohydromonas australica using sucrose and the estimation of fundamental kinetic parameters of PHB fermentation process. The preliminary culture growth inhibition studies were followed by statistical optimization of medium recipe using response surface methodology to increase the PHB production. Later on batch cultivation in a 7-L bioreactor was attempted using optimum concentration of medium components (process variables) obtained from statistical design to identify the batch growth and product kinetics parameters of PHB fermentation. A. australica exhibited a maximum biomass and PHB concentration of 8.71 and 6.24 g/L, respectively in bioreactor with an overall PHB production rate of 0.75 g/h. Bioreactor cultivation studies demonstrated that the specific biomass and PHB yield on sucrose was 0.37 and 0.29 g/g, respectively. The kinetic parameters obtained in the present investigation would be used in the development of a batch kinetic mathematical model for PHB production which will serve as launching pad for further process optimization studies, e.g., design of several bioreactor cultivation strategies to further enhance the biopolymer production.
机译：聚羟基丁酸酯或PHB是一种可生物降解和生物相容的热塑性塑料，在医药，食品包装和组织工程材料中有许多有趣的应用。本研究涉及使用蔗糖提高澳大利亚偶氮氢单胞菌对PHB的产量，并估算PHB发酵过程的基本动力学参数。初步的培养物生长抑制研究之后，采用响应面方法提高PHB产量，对培养基配方进行统计学优化。之后，尝试使用从统计设计中获得的最佳培养基成分浓度（过程变量）在7 L生物反应器中进行培养，以识别PHB发酵的批次生长和产物动力学参数。在生物反应器中，澳大利亚曲霉的最大生物量和PHB浓度分别为8.71和6.24 g / L，总PHB生产率为0.75 g / h。生物反应器培养研究表明，蔗糖的特定生物量和PHB产量分别为0.37和0.29 g / g。本研究中获得的动力学参数将用于开发PHB生产的批量动力学数学模型，该模型将用作进一步工艺优化研究（例如设计几种生物反应器培养策略以进一步提高生物聚合物产量）的启动垫。

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