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首页> 外文期刊>Applied in vitro toxicology. >Evaluation of the Biological Effects of Novel Tobacco Product Vapor Using Two- or Three-Dimensional Culture Systems of Human Bronchial Epithelia
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Evaluation of the Biological Effects of Novel Tobacco Product Vapor Using Two- or Three-Dimensional Culture Systems of Human Bronchial Epithelia

机译：评价生物效应的小说烟草产品使用两个或蒸汽人类的三维培养系统支气管上皮细胞

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Introduction: Because of the rising popularity of novel tobacco products, there has been an increasing interest in the biological effects of these products. This study was performed to investigate the biological effects of vapor from a novel tobacco vapor product (NTV) in comparison with cigarette smoke (CS) from a 3R4F reference cigarette by using two types of in vitro model.Materials and Methods: A two-dimensional (2D) culture system composed of a submerged monolayer of BEAS-2B cells was used to detect cellular oxidative stress responses. A three-dimensional (3D) culture system composed of a pseudostratified air–liquid interface culture of normal human bronchial epithelial cells was used to detect tissue inflammation. In both culture systems, exposures were conducted with an aqueous extract (AqE) of NTV vapor or CS.Results: In the 2D culture system, exposure to CS AqE induced glutathione oxidization and antioxidant response element reporter gene activation. NTV vapor AqE also induced reporter gene activity, but the concentration required for activation was higher than that of CS AqE. In the 3D culture system, exposure to CS AqE increased the secretion of various inflammatory mediators. In contrast, the effect of NTV vapor AqE on tissue inflammation was limited, and only interferon-γ showed a slight increase in expression.Conclusions: Overall, these results demonstrate that NTV vapor has fewer effects on cellular oxidative stress and tissue inflammatory responses as compared with CS in in vitro models using human cells.
机译：简介:因为的日益普及新颖的烟草产品,已经有一个增加兴趣的生物效应这些产品。研究蒸汽的生物效应一种新型烟草蒸汽产品(NTV)相比与香烟(CS)从3 r4f参考通过使用两种类型的体外香烟模型。(2 d)文化系统组成的淹没单层BEAS-2B细胞被用来检测细胞氧化应激反应。三维(3 d)文化系统组成的pseudostratified气液界面文化正常的人类支气管上皮细胞用于检测组织炎症。培养系统,进行了曝光水提物(AqE) NTV蒸汽或CS。在2 d文化系统中,暴露在CS AqE诱导谷胱甘肽氧化和抗氧化剂响应元件报告基因激活。蒸汽AqE也诱导报告基因活动,但激活所需的浓度高于CS AqE。系统,暴露在CS AqE增加了各种炎症介质的分泌。相反,NTV蒸汽AqE对组织的影响炎症是有限的,只有干扰素-γ显示在略有增加表达式。证明NTV蒸汽影响较少组织细胞氧化应激和炎症反应与CS在体外模型用人类细胞。

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