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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Pentameric assembly of the Kv2.1 tetramerization domain
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Pentameric assembly of the Kv2.1 tetramerization domain

机译：Pentameric组装的Kv2.1 tetramerization域

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摘要

The Kv family of voltage-gated potassium channels regulate neuronal excitability. The biophysical characteristics of Kv channels can be matched to the needs of different neurons by forming homotetrameric or heterotetrameric channels within one of four subfamilies. The cytoplasmic tetramerization (T1) domain plays a major role in dictating the compatibility of different Kv subunits. The only Kv subfamily lacking a representative structure of the T1 domain is the Kv2 family. Here, X-ray crystallography was used to solve the structure of the human Kv2.1 T1 domain. The structure is similar to those of other T1 domains, but surprisingly formed a pentamer instead of a tetramer. In solution the Kv2.1 T1 domain also formed a pentamer, as determined by inline SEC-MALS-SAXS and negative-stain electron microscopy. The Kv2.1 T1-T1 interface involves electrostatic interactions, including a salt bridge formed by the negative charges in a previously described CDD motif, and inter-subunit coordination of zinc. It is shown that zinc binding is important for stability. In conclusion, the Kv2.1 T1 domain behaves differently from the other Kv T1 domains, which may reflect the versatility of Kv2.1, which can assemble with the regulatory KvS subunits and scaffold ER-plasma membrane contacts.
机译：千伏的电压门控钾离子通道家族调节神经元兴奋性。Kv渠道可以匹配的特征不同的神经元形成的需要homotetrameric或heterotetrameric通道在一个四个亚科。tetramerization (T1)领域中起着重要作用决定不同Kv的兼容性子单元。T1的代表结构域Kv2家庭。解决人类Kv2.1 T1的结构域。其他T1域,但令人惊讶的是形成了一个五聚物而不是四聚物。Kv2.1 T1领域也形成了一个五聚物,由内联SEC-MALS-SAXS和决定negative-stain电子显微镜。T1-T1接口包括静电交互,包括形成的盐桥先前描述的负电荷CDD主题,inter-subunit协调锌。为稳定。的行为不同于其他Kv T1域,这可能反映Kv2.1的多功能性,可以组装监管千伏子单元和脚手架ER-plasma膜接触。

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