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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Structure and mechanism of the γ-glutamyl-γ-aminobutyrate hydrolase SpuA from Pseudomonas aeruginosa
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Structure and mechanism of the γ-glutamyl-γ-aminobutyrate hydrolase SpuA from Pseudomonas aeruginosa

机译：的结构和机制γ谷酰基-γ-aminobutyrate水解酶的自然铜绿假单胞菌

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摘要

Polyamines are important regulators in all living organisms and are implicated in essential biological processes including cell growth, differentiation and apoptosis. Pseudomonas aeruginosa possesses an spuABCDEFGHI gene cluster that is involved in the metabolism and uptake of two polyamines: spermidine and putrescine. In the proposed γ-glutamylation–putrescine metabolism pathway, SpuA hydrolyzes γ-glutamyl-γ-aminobutyrate (γ-Glu-GABA) to glutamate and γ-aminobutyric acid (GABA). In this study, crystal structures of P. aeruginosa SpuA are reported, confirming it to be a member of the class I glutamine amidotransferase (GAT) family. Activity and substrate-binding assays confirm that SpuA exhibits a preference for γ-Glu-GABA as a substrate. Structures of an inactive H221N mutant were determined with bound glutamate thioester intermediate or glutamate product, thus delineating the active site and substrate-binding pocket and elucidating the catalytic mechanism. The crystal structure of another bacterial member of the class I GAT family from Mycolicibacterium smegmatis (MsGATase) in complex with glutamine was determined for comparison and reveals a binding site for glutamine. Activity assays confirm that MsGATase has activity for glutamine as a substrate but not for γ-Glu-GABA. The work reported here provides a starting point for further investigation of polyamine metabolism in P. aeruginosa.
机译：多胺是重要的监管机构在所有的生活生物和参与是必不可少的生物过程包括细胞生长,分化和细胞凋亡。绿脓杆菌具有spuABCDEFGHI基因簇这是参与的代谢和吸收两种聚胺类:亚精胺和腐胺。提出了γ-glutamylation-putrescine新陈代谢通路,自然水解谷氨酸和γ氨基丁酸(GABA)酸。研究中,铜绿假单胞菌自然的晶体结构报告,确认它的成员吗类我谷氨酰胺amidotransferase(得到)的家庭。活动和substrate-binding化验证实这自然展品偏爱γ-Glu-GABA一个衬底。突变体与绑定谷氨酸测定硫代酸酯中间或谷氨酸产品,因此描述substrate-binding和活性部位口袋里,阐明催化机制。另一个细菌的晶体结构成员类的我从Mycolicibacterium手枪的家庭smegmatis (MsGATase)和谷氨酰胺在复杂确定比较和揭示了吗谷氨酰胺结合位点。确认MsGATase活动谷氨酰胺作为底物而不是γ-Glu-GABA。这里提供了一个起点聚胺代谢的进一步调查p .计数。

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