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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Structure and metal-binding properties of PA4063, a novel player in periplasmic zinc trafficking by Pseudomonas aeruginosa
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Structure and metal-binding properties of PA4063, a novel player in periplasmic zinc trafficking by Pseudomonas aeruginosa

机译：PA4063的结构和性质,小说在周质的锌贩卖铜绿假单胞菌

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摘要

The capability to obtain essential nutrients in hostile environments is a critical skill for pathogens. Under zinc-deficient conditions, Pseudomonas aeruginosa expresses a pool of metal homeostasis control systems that is complex compared with other Gram-negative bacteria and has only been partially characterized. Here, the structure and zinc-binding properties of the protein PA4063, the first component of the PA4063–PA4066 operon, are described. PA4063 has no homologs in other organisms and is characterized by the presence of two histidine-rich sequences. ITC titration detected two zinc-binding sites with micromolar affinity. Crystallographic characterization, performed both with and without zinc, revealed an α/β-sandwich structure that can be classified as a noncanonical ferredoxin-like fold since it differs in size and topology. The histidine-rich stretches located at the N-terminus and between β3 and β4 are disordered in the apo structure, but a few residues become structured in the presence of zinc, contributing to coordination in one of the two sites. The ability to bind two zinc ions at relatively low affinity, the absence of catalytic cavities and the presence of two histidine-rich loops are properties and structural features which suggest that PA4063 might play a role as a periplasmic zinc chaperone or as a concentration sensor useful for optimizing the response of the pathogen to zinc deficiency.
机译：能力获得必需的营养充满敌意的环境中是一项至关重要的技能病原体。铜绿假单胞菌表达的金属体内平衡复杂的控制系统相比之下,其他革兰氏阴性细菌和只有部分的特点。结构和锌结合的属性蛋白质PA4063,第一个组成部分PA4063-PA4066操纵子,。在其他生物,没有同系物特点是两个的存在富含组氨酸序列。两个锌结合网站微摩尔的亲和力。晶体特征,同时执行有和没有锌,透露一个α/β三明治结构,可以分为一个经典之中ferredoxin-like折叠,因为它不同的规模和拓扑。位于n端和之间β3和β4 apo无序的结构,但一些残留成为结构化的锌,有助于协调两个网站之一。锌离子在相对较低的亲和力,没有催化蛀牙和两个的存在问题属性和循环表明PA4063结构特点可能发挥作用作为一个周质的锌女伴或用于浓度传感器优化响应病原体的锌缺乏。

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