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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Evaluation of the data-collection strategy for room-temperature micro-crystallography studied by serial synchrotron rotation crystallography combined with the humid air and glue-coating method
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Evaluation of the data-collection strategy for room-temperature micro-crystallography studied by serial synchrotron rotation crystallography combined with the humid air and glue-coating method

机译：数据收集策略的评估室温micro-crystallography研究串行同步旋转晶体学加上潮湿的空气和涂胶方法

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Synchrotron serial crystallography (SSX) is an emerging data-collection method for micro-crystallography on synchrotron macromolecular (MX) crystallography beamlines. At SPring-8, the feasibility of the fixed-target approach was examined by collecting data using a 2D raster scan combined with goniometer rotation. Results at cryogenic temperatures demonstrated that rotation is effective for efficient data collection in SSX and the method was named serial synchrotron rotation crystallography (SS-ROX). To use this method for room-temperature (RT) data collection, a humid air and glue-coating (HAG) method was developed in which data were collected from polyvinyl alcohol-coated microcrystals fixed on a loop under humidity-controlled air. The performance and the RT data-collection strategy for micro-crystallography were evaluated using microcrystals of lysozyme. Although a change in unit-cell dimensions of up to 1% was observed during data collection, the impact on data quality was marginal. A comparison of data obtained at various absorbed doses revealed that absorbed doses of up to 210 kGy were tolerable in both global and local damage. Although this limits the number of photons deposited on each crystal, increasing the number of merged images improved the resolution. On the basis of these results, an equation was proposed that relates the achievable resolution to the total photon flux used to obtain a data set.
机译：同步串行结晶学(SSX)是一个新兴的数据收集方法micro-crystallography在同步加速器大分子晶体学beamlines (MX)。SPring-8,固定目标的可行性使用方法是研究通过收集数据二维光栅扫描结合测角仪旋转。在低温下显示结果旋转是有效的有效的数据SSX和方法被命名为串行的集合同步旋转晶体学(SS-ROX)。室温(RT)数据的使用这种方法收集、潮湿的空气和涂胶(女巫)收集数据的方法从聚乙烯alcohol-coated微晶核固定的在一个循环空气湿度。性能和RT数据收集策略micro-crystallography评估使用溶菌酶的微晶核。观察单胞尺寸的1%在数据收集过程中,对数据的影响质量是边际。获得了在不同吸收剂量显示吸收剂量的210 kGy的容许了全球及当地的损害。限制了光子的数量在每个沉积水晶,提高融合图像的数量提高了分辨率。结果,一个方程,提出了有关可行的解决总光子通量用于获得一个数据集。

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