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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Structural insights into the interaction of papainlike protease 2 from the alphacoronavirus porcine epidemic diarrhea virus and ubiquitin
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Structural insights into the interaction of papainlike protease 2 from the alphacoronavirus porcine epidemic diarrhea virus and ubiquitin

机译：结构的见解之间的相互作用从alphacoronavirus papainlike蛋白酶2猪流行性腹泻病毒和泛素

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摘要

Porcine epidemic diarrhea is a devastating porcine disease that is caused by the alphacoronavirus porcine epidemic diarrhea virus (PEDV). Like other members of the Coronaviridae family, PEDV encodes a multifunctional papain-like protease 2 (PLP2) that has the ability to process the coronavirus viral polyprotein to aid in RNA replication and antagonize the host innate immune response through cleavage of the regulatory proteins ubiquitin (Ub) and/or interferon-stimulated gene product 15 (ISG15) (deubiquitination and deISGyl-ation, respectively). Because Betacoronavirus PLPs have been well characterized, it was sought to determine how PLP2 from the alphacoronavirus PEDV differentiates itself from its related counterparts. PEDV PLP2 was first biochemically characterized, and a 3.1 A resolution crystal structure of PEDV PLP2 bound to Ub was then solved, providing insight into how Alphacoronavirus PLPs bind to their preferred substrate, Ub. It was found that PEDV PLP2 is a deubiquitinase and readily processes a variety of di-Ub linkages, in comparison with its Betacoronavirus counterparts, which have a narrower range of di-Ub activity but process both Ub and ISG15.
机译：猪流行性腹泻是一种毁灭性的猪alphacoronavirus所造成的疾病猪流行性腹泻病毒(PEDV)。PEDV Coronaviridae家族的其他成员编码一个多功能papain-like蛋白酶2(PLP2)有能力处理冠状病毒病毒RNA多蛋白帮助复制和对抗宿主先天免疫响应通过解理的监管蛋白质泛素(乌兰巴托)和(或)干扰素刺激基因产物15 (ISG15)(deubiquitination和deISGyl-ation,分别)。很好的特点,寻求确定从alphacoronavirus PLP2 PEDV区分本身和它相关的同行。3.1特征,解析晶体结构PEDV PLP2绑定到乌兰巴托如何解决,提供见解Alphacoronavirus PLPs绑定到他们喜欢的衬底,乌兰巴托。deubiquitinase和各种容易进程di-Ub联系,与它相比Betacoronavirus同行,也有一个窄范围的di-Ub活动但过程乌兰巴托和ISG15。

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