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首页> 外文期刊>Acta crystallographica. Section D, Structural biology >Validation, analysis and annotation of cryo-EM structures
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Validation, analysis and annotation of cryo-EM structures

机译：低温电子显微镜的验证、分析和注释结构

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摘要

The process of turning 2D micrographs into 3D atomic models of the imaged macromolecules has been under rapid development and scrutiny in the field of cryo-EM. Here, some important methods for validation at several stages in this process are described. Firstly, how Fourier shell correlation of two independent maps and phase randomization beyond a certain frequency address the assessment of map resolution is reviewed. Techniques for local resolution estimation and map sharpening are also touched upon. The topic of validating models which are either built de novo or based on a known atomic structure fitted into a cryo-EM map is then approached. Map-model comparison using Q-scores and Fourier shell correlation plots is used to assure the agreement of the model with the observed map density. The importance of annotating the model with B factors to account for the resolvability of individual atoms in the map is illustrated. Finally, the timely topic of detecting and validating water molecules and metal ions in maps that have surpassed ~2 A resolution is described.
机译：将二维显微图转化为3 d的过程原子模型成像的大分子在快速发展和审查低温电子显微镜。在这一过程中验证的几个阶段描述。相关的两个独立的地图和阶段随机化超过一定频率的地址评估映射分辨率了。当地的技术估计和决议地图磨也谈及。验证模型的构建德新生,或者是基于一个已知的原子结构安装然后接触到低温电子显微镜图。比较使用Q-scores和傅里叶壳相关情节用于保证协议模型的密度与观察到的地图。注释与B因素模型的重要性考虑到个人的可分解性原子在地图上。及时检测和验证水的话题分子和金属离子的地图超过了~ 2的决议。

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