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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >Analysis of crystalline and solution states of ligand-free spermidine N-acetyltransferase (SpeG) from Escherichia coli
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Analysis of crystalline and solution states of ligand-free spermidine N-acetyltransferase (SpeG) from Escherichia coli

机译：水晶和解决方案的分析ligand-free亚精胺N-acetyltransferase (SpeG)从大肠杆菌

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摘要

Spermidine N-acetyltransferase (SpeG) transfers an acetyl group from acetyl-coenzyme A to an N-terminal amino group of intracellular spermidine. This acetylation inactivates spermidine, reducing the polyamine toxicity that tends to occur under certain chemical and physical stresses. The structure of the SpeG protein from Vibrio cholerae has been characterized: while the monomer possesses a structural fold similar to those of other Gcn5-related N-acetyl-transferase superfamily members, its dodecameric structure remains exceptional. In this paper, structural analyses of SpeG isolated from Escherichia coli are described. Like V. cholerae SpeG, E. coli SpeG forms dodecamers, as revealed by two crystal structures of the ligand-free E. coli SpeG dodecamer determined at 1.75 and 2.9 A resolution. Although both V. cholerae SpeG and E. coli SpeG can adopt an asymmetric open dodecameric state, solution analysis showed that the oligomeric composition of ligand-free E. coli SpeG differs from that of ligand-free V. cholerae SpeG. Based on these data, it is proposed that the equilibrium balance of SpeG oligomers in the absence of ligands differs from one species to another and thus might be important for SpeG function.
机译：亚精胺N-acetyltransferase (SpeG)转移乙酰基acetyl-coenzyme一个氨基端氨基的细胞内亚精胺。亚精胺,降低聚胺的毒性往往发生在某些化学和身体压力。蛋白质从霍乱弧菌特点:单体具有结构折叠与其他相似Gcn5-related N-acetyl-transferase总科成员,其dodecameric结构仍然存在例外。SpeG分离的大肠杆菌描述。dodecamers形式,揭示了两个晶体ligand-free SpeG大肠杆菌的结构dodecamer决定在1.75和2.9决议。杆菌SpeG可以采用非对称开放分析表明,dodecameric状态,解决方案的低聚物的成分ligand-free大肠杆菌SpeG有别于ligand-free霍乱弧菌SpeG。平衡平衡SpeG寡聚物没有配体与一个物种另一个,从而可能对SpeG非常重要函数。

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