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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >Microtubule architecture in vitro in vitro and in cells revealed by cryo‐electron tomography
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Microtubule architecture in vitro in vitro and in cells revealed by cryo‐electron tomography

机译：微管体外体外和架构细胞显示,低温电子断层扫描

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The microtubule cytoskeleton is involved in many vital cellular processes. Microtubules act as tracks for molecular motors, and their polymerization and depolymerization can be harnessed to generate force. The structures of microtubules provide key information about the mechanisms by which their cellular roles are accomplished and the physiological context in which these roles are performed. Cryo‐electron microscopy allows the visualization of in vitro ‐polymerized microtubules and has provided important insights into their overall morphology and the influence of a range of factors on their structure and dynamics. Cryo‐electron tomography can be used to determine the unique three‐dimensional structure of individual microtubules and their ends. Here, a previous cryo‐electron tomography study of in vitro ‐polymerized GMPCPP‐stabilized microtubules is revisited, the findings are compared with new tomograms of dynamic in vitro and cellular microtubules, and the information that can be extracted from such data is highlighted. The analysis shows the surprising structural heterogeneity of in vitro ‐polymerized microtubules. Lattice defects can be observed both in vitro and in cells. The shared ultrastructural properties in these different populations emphasize the relevance of three‐dimensional structures of in vitro microtubules for understanding microtubule cellular functions.
机译：微管细胞骨架参与很多重要的细胞过程。跟踪分子马达,和他们的聚合与解聚作用利用生成力。微管提供关键的信息细胞角色的机制完成和生理环境这些角色执行。显微镜使体外的可视化聚合的微管和提供了重要的洞察他们的整体形态和一系列因素对他们的影响的结构和动力学。可以用来确定独特的三维结构等人微管及其目的。低温电子断层扫描研究体外量聚合GMPCPP量稳定微管重新审视,发现比较新x线断层照片动态体外和细胞微管,可以的信息从这些数据中提取突出显示。分析显示了惊人的结构异质性的体外的聚合微管。在体外和细胞。在这些不同的超微结构的属性人群中强调的相关性三维结构的体外微管对理解微管细胞的功能。

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