...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >Approaches to altering particle distributions in cryo‐electron microscopy sample preparation
【24h】

Approaches to altering particle distributions in cryo‐electron microscopy sample preparation

机译：方法改变粒子的分布低温电子显微镜样品制备

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cryo‐electron microscopy (cryo‐EM) can now be used to determine high‐resolution structural information on a diverse range of biological specimens. Recent advances have been driven primarily by developments in microscopes and detectors, and through advances in image‐processing software. However, for many single‐particle cryo‐EM projects, major bottlenecks currently remain at the sample‐preparation stage; obtaining cryo‐EM grids of sufficient quality for high‐resolution single‐particle analysis can require the careful optimization of many variables. Common hurdles to overcome include problems associated with the sample itself (buffer components, labile complexes), sample distribution (obtaining the correct concentration, affinity for the support film), preferred orientation, and poor reproducibility of the grid‐making process within and between batches. This review outlines a number of methodologies used within the electron‐microscopy community to address these challenges, providing a range of approaches which may aid in obtaining optimal grids for high‐resolution data collection.
机译：低温电子显微镜(低温检测——EM)应承担的现在可以使用确定高分辨率结构各种各样的生物信息标本。主要是通过显微镜和的发展探测器,通过进步图像处理软件。单粒子应承担的低温高EM项目专业目前处于瓶颈样品检测准备阶段;高分辨率的足够的质量单粒子分析需要小心许多变量的优化。克服包括相关问题样品本身(缓冲组件,不稳定复合物),样本分布(获得正确的浓度,亲和力的支持膜)、择优取向和贫穷再现性地理网格的制作过程和批次之间。数量的方法中使用电子显微镜社区解决这些挑战,提供了一个范围的方法可以帮助获得最佳网格吗高分辨率数据收集。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号