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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >A pipeline approach to single‐particle processing in RELION RELION
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A pipeline approach to single‐particle processing in RELION RELION

机译：一个管道方法单一粒子处理在RELION RELION

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摘要

The formal concept of a workflow to single‐particle analysis of cryo‐electron microscopy (cryo‐EM) images in the RELION program is described. In this approach, the structure‐determination process is considered as a graph, where intermediate results in the form of images or metadata are the vertices, and different functionalities of the program are the edges. The new implementation automatically logs all user actions, facilitates file management and disk cleaning, and allows convenient browsing of the history of a project. Moreover, new functionality to iteratively execute consecutive jobs allows on‐the‐fly image processing, which will lead to more efficient data acquisition by providing faster feedback on data quality. The possibility of exchanging data‐processing procedures among users will contribute to the development of standardized image‐processing procedures, and hence increase accessibility for new users in this rapidly expanding field.
机译：正式意义上的工作流单颗粒分析等低温电子低温显微镜(EM)应承担RELION项目图片是描述。结构确定过程被认为是一个图,中间结果的形式图像或元数据的顶点,不同的功能的程序边缘。所有用户操作,文件管理和促进磁盘清理,并允许方便的浏览一个项目的历史。连续迭代执行的功能工作允许作业高动态图像处理将导致更有效的数据采集提供更快的数据质量反馈。交换的数据量处理的可能性用户将为程序发展标准化图像检测处理程序,因此增加的可访问性新用户在这个快速扩张的领域。

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