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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >Transmission electron microscopy for the evaluation and optimization of crystal growth
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Transmission electron microscopy for the evaluation and optimization of crystal growth

机译：透射电子显微镜的评价和优化晶体生长

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摘要

The crystallization of protein samples remains the most significant challenge in structure determination by X-ray crystallography. Here, the effectiveness of transmission electron microscopy (TEM) analysis to aid in the crystallization of biological macromolecules is demonstrated. It was found that the presence of well ordered lattices with higher order Bragg spots, revealed by Fourier analysis of TEM images, is a good predictor of diffraction-quality crystals. Moreover, the use of TEM allowed (i) comparison of lattice quality among crystals from different conditions in crystallization screens; (ii) the detection of crystal pathologies that could contribute to poor X-ray diffraction, including crystal lattice defects, anisotropic diffraction and crystal contamination by heavy protein aggregates and nanocrystal nuclei; (iii) the qualitative estimation of crystal solvent content to explore the effect of lattice dehydration on diffraction and (iv) the selection of high-quality crystal fragments for microseeding experiments to generate reproducibly larger sized crystals. Applications to X-ray free-electron laser (XFEL) and micro-electron diffraction (microED) experiments are also discussed.
机译：蛋白质结晶的样品仍然是结构的最重要的挑战通过x射线结晶学的决心。透射电子显微镜法的有效性(TEM)分析援助的结晶生物大分子。发现的存在以及有序的晶格与高阶布拉格的斑点,揭示了傅里叶分析的TEM图像,是一个很好的预测diffraction-quality晶体。此外,允许使用TEM (i)比较晶体中晶格质量不同在屏幕结晶条件;检测的水晶病态导致贫穷的x射线衍射,包括晶格缺陷,各向异性衍射和水晶污染重的蛋白质聚合物和纳米晶体核;定性评价结晶溶剂的内容探索晶格脱水的效果衍射和(iv)的选择高质量的水晶碎片microseeding实验生成可再生产地更大的尺寸晶体。激光(XFEL)和微电子衍射(微)实验进行了讨论。

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