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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >DNA polymorphism in crystals: three stable conformations for the decadeoxynucleotide d(GCATGCATGC)
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DNA polymorphism in crystals: three stable conformations for the decadeoxynucleotide d(GCATGCATGC)

机译：DNA多态性在晶体:三个稳定的构象decadeoxynucleotided (GCATGCATGC)

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摘要

High-resolution structures of DNA fragments determined using X-ray crystallography or NMR have provided descriptions of a veritable alphabet of conformations. They have also shown that DNA is a flexible molecule, with some sequences capable of adopting two different structures. Here, the first example is presented of a DNA fragment that can assume three different and distinct conformations in crystals. The decanucleotide d(GCATGCATGC) was previously reported to assume a single-stranded double-fold structure. In one of the two crystal structures described here the decamer assumes both the double-fold conformation and, simultaneously, the more conventional B-type double-helical structure. In the other crystal the sequence assumes the A-type double-helical conformation. These results, taken together with CD spectra, which were recorded as the decamer was titrated against four metal ions and spermine, indicate that the molecule may exist as a mixed population of structures in solution. Small differences in the environmental conditions, such as the concentration of metal ion, may decide which of these crystallizes out. The results also support the idea that it may be possible for DNA to change its structure to suit the binding requirements of proteins or drugs.
机译：高分辨率结构的DNA片段决定使用x射线晶体学、核磁共振提供了名副其实的描述字母的构象。DNA是一个灵活的分子,与一些序列采用两种不同的能力结构。可以假设三种不同的DNA片段和不同的晶体构象。decanucleotide d (GCATGCATGC)之前据报道,假设一个单链双重结构。这里描述decamer假设的双重构造,同时,更传统的b型双螺旋结构。假设a类型双螺旋构象。这些结果,结合CD光谱,这记录decamer滴定对四种金属离子和精胺,指示作为混合分子可能存在人口结构的解决方案。环境条件,如金属离子的浓度,可以决定的这些结晶出来。的想法是可能的DNA改变它的结构,以适应绑定蛋白质或药物的需求。

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