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首页> 外文期刊>Acta crystallographica. Section D, Structural biology. >Thermal contraction of aqueous glycerol and ethylene glycol solutions for optimized protein-crystal cryoprotection
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Thermal contraction of aqueous glycerol and ethylene glycol solutions for optimized protein-crystal cryoprotection

机译：水甘油和热收缩乙二醇为优化解决方案测定晶体cryoprotection

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摘要

The thermal contraction of aqueous cryoprotectant solutions on cooling to cryogenic temperatures is of practical importance in protein cryocrystallography and in biological cryopreservation. In the former case, differential contraction on cooling of protein molecules and their lattice relative to that of the internal and surrounding solvent may lead to crystal damage and the degradation of crystal diffraction properties. Here, the amorphous phase densities of aqueous solutions of glycerol and ethylene glycol at T = 77 K have been determined. Densities with accuracies of <0.5% to concentrations as low as 30%(w/v) were determined by rapidly cooling drops with volumes as small as 70 pl, assessing their optical clarity and measuring their buoyancy in liquid nitrogen-argon solutions. The use of these densities in contraction matching of internal solvent to the available solvent spaces is complicated by several factors, most notably the exclusion of cryoprotectants from protein hydration shells and the expected deviation of the contraction behavior of hydration water from bulk water. The present methods and results will assist in developing rational approaches to cryoprotection and an understanding of solvent behavior in protein crystals.
机译：水冷冻保护剂的热收缩在冷却到低温下的解决方案实际意义的蛋白质cryocrystallography和生物低温贮藏。微分冷却收缩的蛋白质分子和晶格相对的内部和周围的溶剂可能导致晶体破坏和退化的晶体衍射特性。密度的甘油和水的解决方案乙二醇在T = 77 K已经确定。密度的精度小于0.5%浓度低至30% (w / v)测定迅速冷却的滴体积尽可能小70 . pl,评估他们的光学清晰度和测量液体nitrogen-argon的浮力解决方案。收缩匹配的内部溶剂溶剂可用空间是复杂的有几个因素,尤其是排除从蛋白质水化壳和冷冻保护剂收缩的预期偏差水化水从大体积的水的行为。方法和结果将协助cryoprotection发展理性的方法和溶剂行为的理解蛋白质晶体。

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