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首页> 外文期刊>Journal of Cellular Physiology >Neural differentiation of human adipose‐derived mesenchymal stem cells induced by glial cell conditioned media
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Neural differentiation of human adipose‐derived mesenchymal stem cells induced by glial cell conditioned media

机译：神经分化的人类脂肪量间充质干细胞诱导的神经胶质细胞的媒体

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Adipose‐derived mesenchymal stem cells (ASCs) may transdifferentiate into cells belonging to mesodermal, endodermal, and ectodermal lineages. The aim of this study was to verify whether a neural differentiation of ASCs could be induced by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). ASCs were isolated from the stromal vascular fraction of adipose tissue and expanded for 2–3 passages. They were then cultured in OEC‐CM or SC‐CM for 24?hr or 7 days. At each stage, the cells were tested by immunocytochemistry and flow cytometer analysis to evaluate the expression of typical neural markers such as Nestin, PGP 9.5, MAP2, Synapsin I, and GFAP. Results show that both conditioned media induced similar positive effects, as all tested markers were overexpressed, especially at day 7. Overall, an evident trend toward neuronal or glial differentiation was not clearly detectable in many cases. Nevertheless, a higher tendency toward a neuronal phenotype was recognized for OEC‐CM (considering MAP2 increases). On the other hand, SC‐CM would be responsible for a more marked glial induction (considering GFAP increases). These findings confirm that environmental features can induce ASCs toward a neural differentiation, either as neuronal or glial elements. Rather than supplementing the culture medium by adding chemical agents, a “more physiological” condition was obtained here by means of soluble factors (cytokines/growth factors) likely released by glial cells. This culture strategy may provide valuable information in the development of cell‐based therapeutic approaches for pathologies affecting the central/peripheral nervous system.
机译：脂肪量间充质干细胞(对asc)可能派生而来属于transdifferentiate进入细胞中胚层、内胚层和外胚层的血统。本研究的目的是验证是否对asc神经分化的诱导的条件培养基(CM)获得文化(近年)或嗅鞘细胞雪旺细胞(SCs)。间质血管脂肪组织的一部分扩大2 - 3段。培养在OEC高24厘米或者SC厘米吗?在每个阶段,细胞进行了测试免疫细胞化学和流式细胞分析仪分析评估的表达典型的神经标记如巢蛋白、PGP 9.5、MAP2 Synapsin我和GFAP。媒体诱导类似的积极作用,因为所有测试标记中,特别是在7天。或胶质分化不明显在许多情况下可检测。倾向一个神经细胞表型公认为OEC CM(考虑到MAP2增加)。负责神经胶质感应更明显(考虑GFAP增加)。确认环境功能可以诱导对asc向神经分化,要么神经元或神经胶质元素。补充添加的培养基化学药剂,“生理”条件通过可溶性因子得到吗(细胞因子和生长因子)可能发布的神经胶质细胞。发展的有价值的信息细胞治疗病态的基础方法影响中央/周围神经系统。

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