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首页> 外文期刊>Journal of Cellular Physiology >RRM2 RRM2 promotes the progression of human glioblastoma
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RRM2 RRM2 promotes the progression of human glioblastoma

机译：RRM2 RRM2促进人类的发展胶质母细胞瘤

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摘要

Glioblastoma pathogenesis is related to multiple processes that affected by dozens of regulatory factors, but the potential underlying factors regulating glioblastoma progression remains unclear. The goal of this research was to determine how the ribonucleotide reductase M2 subunit ( RRM2 ) influenced proliferation, invasion, migration, and apoptosis of human glioblastoma cells. The level of proliferation of human glioblastoma cells was measured through CCK8, colony formation assay and immunofluorescence stains. Flow cytometry (FCM), wound healing, and transwell assays were conducted to detect cell apoptosis, migration, and invasion. Apoptotic level of cells and invasion‐related expression of protein were measured by Western blot. Xenograft tumor model was established to confirm effect of RRM2 on the proliferation of human glioblastoma cells in vivo. Silencing RRM2 inhibited proliferation, invasion, and migration of glioblastoma cells whereas enhanced apoptosis rate. Overexpressing RRM2 promoted proliferation, migration and invasion but suppressed apoptosis. In vivo, Overexpressing RRM2 accelerated the tumor growth in glioblastoma cells. The present study illustrated that RRM2 was overexpressed in human glioblastoma cells. RRM2 promoted proliferation, migration, and invasion but inhibited apoptosis of human glioblastoma cells.
机译：胶质母细胞瘤发病机制到多个相关过程受许多监管因素,但潜在的潜在因素调节胶质母细胞瘤进展仍然不清楚。确定核苷酸还原酶的平方米亚基(RRM2)增殖的影响,入侵、迁移和细胞凋亡的人类胶质母细胞瘤细胞。人类胶质母细胞瘤细胞测量CCK8、集落形成试验免疫荧光染色。伤口愈合,transwell化验检测细胞凋亡进行迁移,和入侵。入侵检测相关蛋白的表达用免疫印迹。成立的RRM2确认的效果呢人类胶质母细胞瘤细胞的增殖vivo入侵,胶质母细胞瘤细胞的迁移而增强的细胞凋亡率。RRM2促进增殖、迁移和入侵,但抑制细胞凋亡。Overexpressing RRM2加速了肿瘤的生长在胶质母细胞瘤细胞。说明RRM2在人类中胶质母细胞瘤细胞。迁移和入侵,但抑制细胞凋亡人类胶质母细胞瘤细胞。

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