...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >A new gold standard approach to characterize the transport of Si across cell membranes in animals
【24h】

A new gold standard approach to characterize the transport of Si across cell membranes in animals

机译：一个新的黄金标准的方法来描述如果跨细胞膜的运输动物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Silicon (Si) is increasingly recognized as an essential trace element in animals, especially since the identification of mammalian Si transport systems and Si responsive genes not long ago. During many years, however, efforts to gain substantial insight into the biological role of this element in animals have achieved partial success due in part to the unavailability of validated protocols to study Si movement across biological membranes. To circumvent such limitations, we have developed a general transport assay in which cellular Si content was determined by automated electrothermal atomic absorption spectrophotometry. We have found this assay to provide great analytic sensitivity with Si detection thresholds of less than 1?μM, that is, below or very close to the concentration range of animal cells. We have also found this assay to provide valid and cost‐effective determinations in Si transport studies while requiring workable quantities of samples. The protocol described here should thus become gold standard toward accelerated progress in the field of Si transport.
机译：硅(Si)越来越被认为是一个动物必需的微量元素,特别是由于哺乳动物Si的识别运输系统和Si反应基因很久以前。获得大量的洞察的生物作用在动物身上取得了部分的元素成功的部分原因是不可用的验证协议研究如果运动生物膜。的局限性,我们开发了一种通用运输细胞如果内容的分析由自动电热原子吸收分光光度法。提供巨大的解析灵敏度分析如果检测阈值小于1 ?是,非常接近或者低于浓度动物细胞的范围。分析提供有效的和成本有效的如果运输研究决定需要切实可行的数量的样品。这里描述的协议应成为黄金标准对加速进展如果运输。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号