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首页> 外文期刊>Journal of Cellular Physiology >Establishment of an in vitro organoid model of dermal papilla of human hair follicle
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Establishment of an in vitro organoid model of dermal papilla of human hair follicle

机译：建立体外瀑样模型的人的毛囊真皮乳头

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Human hair dermal papilla (DP) cells are specialized mesenchymal cells that play a pivotal role in hair regeneration and hair cycle activation. The current study aimed to first develop three-dimensional (3D) DP spheroids (DPS)with or without a silk-gelatin (SG) microenvironment, which showed enhanced DP-specific gene expression, resulting in enhanced extracellular matrix (ECM) production compared with a monolayer culture. We tested the feasibility of using this DPS model for drug screening by using minoxidil, which is a standard drug for androgenic alopecia. Minoxidil-treated DPS showed enhanced expression of growth factors and ECM proteins. Further, an attempt has been made to establish an in vitro 3D organoid model consisting of DPS encapsulated by SG hydrogel and hair follicle (HF) keratinocytes and stem cells. This HF organoid model showed the importance of structural features, cell-cell interaction, and hypoxia akin to in vivo HF. The study helped to elucidate the molecular mechanisms to stimulate cell proliferation, cell viability, and elevated expression of HF markers as well as epithelial-mesenchymal crosstalks, demonstrating high relevance to human HF biology. This simple in vitro DP organoid model system has the potential to provide significant insights into the underlying mechanisms of HF morphogenesis, distinct molecular signals relevant to different stages of the hair cycle, and hence can be used for controlled evaluation of the efficacy of new drug molecules.
机译：人类头发真皮乳头细胞(DP)专业间充质细胞发挥的关键在头发和头发再生循环中的作用激活。开发三维(3 d) DP球状体(DPS)有或没有一个silk-gelatin (SG)微环境,显示增强DP-specific基因表达,导致增强细胞外基质(ECM)的生产而单层培养。使用这个药物DPS模型的可行性筛选通过使用米诺地尔,这是一个标准雄性脱发的药物。DPS显示增强的生长因子的表达和ECM蛋白质。建立体外3 d制作瀑样模型组成的DPS SG水凝胶和封装毛囊(高频)角化细胞和干细胞。这种高频瀑样模型显示的重要性结构特点、信息交互和缺氧类似于体内高频。阐明刺激的分子机制细胞增殖、细胞生存能力和提升高频标记的表达epithelial-mesenchymal相声,展示人类生物学高频高相关性。体外DP瀑样模型系统可能提供重要的见解高频形态发生的潜在机制,不同的分子信号相关的不同毛发周期的阶段,因此可以使用新控制评价的有效性药物分子。

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