Promotion of the prehierarchical follicle growth by postovulatory follicles involving PGE(2)-EP2 signaling in chickens

Lin Xin; Liu Xingting; Guo ChangquanLiu MengqiMi YulingZhang Caiqiao

摘要

The postovulatory follicle (POF) in birds is an enigmatic structure, the function of which remains largely unknown. Previous studies on chickens have shown that removal of POFs leads to the postponement of oviposition and the disturbance of broody behavior. One suggestion is that POFs may secrete some crucial hormones or cytokines to act on reproductive organs. However, such secretions and their specific target organs remain to be identified. Here, we investigate the putative functions of POFs in promoting the development of prehierarchical follicles in chickens and explore the possible signaling mechanisms controlling these processes. Results show that POFs express steroidogenic acute regulatory protein (STAR), cholesterol side-chain cleavage enzyme (CYP11A1), cyclooxygenase 1 (COX1), and COX2 in granulosa cells (GCs), and, most notably, that POF1 produces more prostaglandin E2 (PGE(2)) or prostaglandin F2 than do the F1 follicle or the other POFs. Using coculture systems, we also found that POF1 or GCs from POF1 (POF1-GCs) significantly promote the proliferation of theca externa cells of small white follicles (SWFs, one phase of the prehierarchical follicle). Treatment with PGE(2) significantly facilitates theca externa cell proliferation in SWFs. This POF-stimulating effect on SWF growth was prevented by treatment with indomethacin (COX inhibitor) or TG6-10-1 (PGE(2) type 2 receptor [EP2] antagonist). Therefore, POF1 may secrete PGE(2) to stimulatethe progression of SWF by PGE(2)-EP2 signaling. These results indicate that POF1 may serve as a transient supplementary endocrine gland in the chicken ovary that stimulates the development of the prehierarchical follicles through PGE(2)-EP2 signaling.

机译：postovulatory卵泡(POF)鸟是一个神秘的结构,它的功能在很大程度上仍是个未知数。鸡已经表明,pof导致产卵和推迟多产的行为的干扰。pof可能分泌激素或一些至关重要的细胞因子作用于生殖器官。这些分泌物及其特定的靶器官仍有待确定。假定的pof的促进功能prehierarchical毛囊的发展鸡和探索可能的信号机制控制这些过程。表明,pof表达steroidogenic急性调节蛋白(明星),胆固醇侧链裂解酶(CYP11A1),环氧酶1(COX1), COX2在颗粒细胞(GCs),最值得注意的是,POF1产生更多前列腺素E2(铂族元素(2))或前列腺素F2比F1卵泡pof。coculture系统,我们还发现POF1或gc从POF1 (POF1-GCs)显著提升膜外扩散的细胞小白毛囊(主权财富基金,一个阶段的prehierarchical滤泡)。显著促进细胞外壁外在主权财富基金扩散。对主权财富基金的增长的影响被治疗预防吲哚美辛(COX抑制剂)或TG6-10-1(铂族元素(2)2型受体(EP2)拮抗剂)。因此,POF1可能分泌铂族元素(2)stimulatethe进展SWF的铂族元素(2)-EP2信号。作为一个临时补充内分泌在鸡卵巢,刺激腺体prehierarchical毛囊的发展通过铂族元素(2)-EP2信号。

Promotion of the prehierarchical follicle growth by postovulatory follicles involving PGE(2)-EP2 signaling in chickens

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