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首页> 外文期刊>Journal of Cellular Physiology >Spheroids from adipose-derived stem cells exhibit an miRNA profile of highly undifferentiated cells
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Spheroids from adipose-derived stem cells exhibit an miRNA profile of highly undifferentiated cells

机译：从脂肪中提取干细胞具有球状体一个高度未分化细胞的microrna的概要文件

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Two-dimensional (2D) cell cultures have been extensively used to investigate stem cell biology, but new insights show that the 2D model may not properly represent the potential of the tissue of origin. Conversely, three-dimensional cultures exhibit protein expression patterns and intercellular junctions that are more representative of their in vivo condition. Multiclonal cells that grow in suspension are defined as spheroids, and we have previously demonstrated that spheroids from adipose-derived stem cells (S-ASCs) displayed enhanced regenerative capability. With the current study, we further characterized S-ASCs to further understand the molecular mechanisms underlying their stemness properties. Recent studies have shown that microRNAs (miRNAs) are involved in many cellular mechanisms, including stemness maintenance and proliferation, and adipose stem cell differentiation. Most studies have been conducted to identify a specific miRNA profile on adherent adipose stem cells, although little is still known about S-ASCs. In this study, we investigate for the first time the miRNA expression pattern in S-ASCs compared to that of ASCs, demonstrating that cell lines cultured in suspension show a typical miRNA expression profile that is closer to the one reported in induced pluripotent stem cells. Moreover, we have analyzed miRNAs that are specifically involved in two distinct moments of each differentiation, namely early and late stages of osteogenic, adipogenic, and chondrogenic lineages during long-term in vitro culture. The data reported in the current study suggest that S-ASCs have superior stemness features than the ASCs and they represent the true upstream stem cell fraction present in adipose tissue, relegating their adherent counterparts.
机译：二维(2 d)细胞培养广泛用于干细胞研究生物,但新见解表明2 d模型可能不正确地代表的潜力组织的起源。文化展览和蛋白表达模式细胞间连接的更多代表他们体内的状况。Multiclonal细胞在悬浮生长定义为球状体,我们以前证明了从脂肪细胞球状体干细胞(S-ASCs)增强显示再生能力。我们进一步S-ASCs进一步表征理解背后的分子机制他们具备干细胞特性。表明小分子核糖核酸(microrna)参与许多细胞机制,包括具备干细胞维护和扩散,脂肪干细胞细胞分化。进行了识别一个特定的microrna的概要文件附着脂肪干细胞,虽然很少还知道S-ASCs。首次调查microrna相比,S-ASCs表达式模式的对asc,证明细胞株培养悬浮显示一个典型的microrna的表达接近报告的一个概要文件诱导多能干细胞。分析具体参与的microrna两个不同时刻的分化,即成骨的早期和晚期阶段,脂肪形成的,期间chondrogenic血统长期体外培养。目前的研究表明,S-ASCs对asc比他们优越具备干细胞特性代表真正的上游干细胞分数存在于脂肪组织,把他们附着。

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