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首页> 外文期刊>Journal of Cellular Physiology >Endocytosed factor V is trafficked to CD42b(+) proplatelet extensions during differentiation of human umbilical cord blood-derived megakaryocytes
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Endocytosed factor V is trafficked to CD42b(+) proplatelet extensions during differentiation of human umbilical cord blood-derived megakaryocytes

机译：V是贩卖到CD42b内源性因素(+)在分化proplatelet扩展人类脐带血液巨核细胞

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Plasma- and platelet-derived factor Va are essential for thrombin generation catalyzed by the prothrombinase complex; however, several observations demonstrate that the platelet-derived cofactor, which is formed following megakaryocyte endocytosis and modification of the plasma procofactor, factor V, is more hemostatically relevant. Factor V endocytosis, as a function of megakaryocyte differentiation and proplatelet formation, was assessed by flow cytometry and microscopy in CD34(+) hematopoietic progenitor cells isolated from human umbilical cord blood and cultured for 12 days in the presence of cytokines to induce ex vivo differentiation into megakaryocytes. Expression of an early marker of megakaryocyte differentiation, CD41, endocytosis of factor V, and the percentage of CD41(+) cells that endocytosed factor V increased from days 6 to 12 of differentiation. In contrast, statistically significant decreases in expression of the stem cell marker, CD34, and in the percentage of CD34(+) cells that endocytosed factor V were observed. A statistically significant increase in the expression of CD42b, a late marker of megakaryocyte differentiation, was also observed over time, such that by Day 12, all CD42b(+) cells endocytosed factor V and expressed CD41. This endocytosed factor V was trafficked to proplatelet extensions and was localized in a punctate pattern in the cytoplasm consistent with its storage in -granules. In conclusion, loss of CD34 and expression of CD42b define cells capable of factor V endocytosis and trafficking to proplatelet extensions during differentiation of megakaryocytes ex vivo from progenitor cells isolated from umbilical cord blood.
机译：血浆和血小板源因素Va对于凝血酶生成催化prothrombinase复杂;观测表明,血小板源辅因子,这就形成了随着巨核细胞内吞作用和改性的等离子procofactor因子V,hemostatically相关。巨核细胞的内吞作用,作为一个函数分化和proplatelet形成流式细胞仪和显微镜评估造血祖细胞CD34(+)孤立从人类脐带血和培养12天的细胞因子诱导的前女友体内分化成巨核细胞。巨核细胞的早期标志物的表达分化、CD41 V内吞作用的因素,和CD41的百分比(+)细胞内源性因素6到12 V从天的分化。干细胞的表达显著下降细胞标记、CD34和的百分比CD34(+)细胞内源性因子V观察到。CD42b的表达,一个迟到的标志巨核细胞分化,也观察到随着时间的推移,这样白天12日所有CD42b (+)V和表达CD41细胞内源性因素。这种内源性因子V是贩卖proplatelet扩展和在一个局部细胞质中的点状的模式一致颗粒的存储。CD42b定义细胞CD34和表达能力V内吞作用和贩卖的因素在分化proplatelet扩展巨核细胞祖细胞体外从脐血中分离出来。

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