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首页> 外文期刊>Journal of Cellular Physiology >Transdifferentiation of human gingival mesenchymal stem cells into functional keratinocytes by Acalypha indica in three-dimensional microenvironment
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Transdifferentiation of human gingival mesenchymal stem cells into functional keratinocytes by Acalypha indica in three-dimensional microenvironment

机译：人类牙龈间叶细胞的分化转移干细胞功能的角化细胞Acalypha籼稻在三维微环境

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Gingival tissue is reportedly a promising, easily accessible, abundant resource of mesenchymal stem cells (MSC) for use in various tissue engineering strategies. Human gingival MSC (HGMSCs) were successfully isolated from gingival tissue and characterized. To analyze in a two-dimensional form, HGMSCs were cultured with basal medium and induced with 25 mu g/ml of Acalypha indica. Quantitative real-time polymerase chain reaction (qPCR) and western blot analysis showed the presence of keratinocyte-specific markers, including cytokeratin-5 and involucrin. To further assess its capability for stratification akin to human keratinocytes, HGMSCs were encapsulated in a HyStem((R))-HP Cell Culture Scaffold Kit and cultured in the presence of A. indica. Calcein AM staining indicated that the HyStem((R))-HP Scaffold Kit has excellent biocompatibility. Immunofluorescence and qPCR analysis revealed the presence of keratinocyte-specific markers. The study concluded that the three-dimensional microenvironment is a novel method for inducing epidermal differentiation of HGMSCs to engineer epidermal substitutes with the help of A. indica, which provides an alternative strategy for skin tissue engineering.
机译：据说是一个有前途的牙龈组织,容易交通便利,资源丰富的间充质干细胞细胞(MSC)用于各种组织工程策略。成功从牙龈组织和孤立为特征。形式,HGMSCs与基础培养基和培养诱导Acalypha籼稻25μg / ml。定量实时聚合酶链反应(qPCR)和免疫印迹分析显示keratinocyte-specific标记,包括cytokeratin-5和involucrin。进一步评估其能力分层类似于人类的角化细胞,HGMSCs封装在一个HyStem ((R))惠普细胞培养支架装备和培养的存在。籼稻。HyStem ((R))惠普支架装备好生物相容性。分析发现的存在keratinocyte-specific标记。得出三维微环境是一种新型诱导的方法表皮分化HGMSCs工程师表皮替代物a .籼稻的帮助下为皮肤提供另外一个策略组织工程。

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