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首页> 外文期刊>Journal of Cellular Physiology >ESRRB plays a crucial role in the promotion of porcine cell reprograming
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ESRRB plays a crucial role in the promotion of porcine cell reprograming

机译：ESRRB推广起着至关重要的作用猪细胞重新编程

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摘要

The estrogen‐related receptor b (ESRRB) is an orphan nuclear receptor and targets many genes involved in self‐renewal and pluripotency. In mouse ES cells, overexpression of ESRRB can maintain LIF‐independent self‐renewal in the absence of Nanog. However, the fundamental features of porcine ESRRB remain elusive. In this study, we revealed the expression profiles of ESRRB in both porcine pluripotent stem cells and early stage embryos and dissected the functional domains of ESRRB protein to prove that ESRRB is a key transcription factor that enhanced porcine pluripotent gene activation. Addition of ESRRB into the cocktail of core pluripotent factors Oct4, Sox2, Klf4, and c‐Myc (OSKM?+?E) could significantly enhance the reprograming efficiency and the formation of alkaline phosphatase positive colonies. Conversely, knockdown of ESRRB in piPSCs significantly reduced the expression level of pluripotent genes, minimized the alkaline phosphatase activity, and initiated the porcine induced pluripotent stem cell differentiation. Therefore, porcine ESRRB is a crucial transcription factor to improve the self‐renewal of piPSCs.
机译：雌激素量相关受体b (ESRRB)是一个孤儿核受体和许多基因的目标参与自我更新和多潜能。老鼠胚胎干细胞,过度的ESRRB即可维持生活还是独立自我的更新缺乏Nanog。猪的特点ESRRB仍然是难以捉摸的。研究中,我们显示的表达谱ESRRB猪多能干细胞和早期胚胎和切割功能域的ESRRB证明ESRRB是蛋白质关键的转录因子,增强猪多能性基因激活。核心的鸡尾酒多能因素Oct4、Sox2 Klf4 c Myc应承担(OSKM + ? E)显著提高重组效率和碱性磷酸酶的形成积极的殖民地。在piPSCs显著降低表达式多能性基因的水平,最小化碱性磷酸酶活性,并且启动了猪诱导多能干细胞分化。至关重要的转录因子来改善自我piPSCs续期。

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