...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >The Fam50a positively regulates ameloblast differentiation via interacting with Runx2
【24h】

The Fam50a positively regulates ameloblast differentiation via interacting with Runx2

机译：Fam50a积极调节造釉细胞通过与Runx2交互分化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Differentiated ameloblasts secret enamel matrix proteins such as amelogenin, ameloblastin, and enamelin. Expression levels of these proteins are regulated by various factors. To find a new regulatory factor for ameloblast differentiation, we performed 2D‐PAGE analysis using mouse ameloblast lineage cell line (mALCs) cultured with mineralizing medium. Of identified proteins, family with sequence similarity 50 member A (Fam50a) was significantly increased during differentiation of mALCs. Fam50a protein was also highly expressed in secretory ameloblasts of mouse tooth germs. In mALCs cultures, forced expression of Fam50a up‐regulated the expression of enamel matrix protein genes such as amelogenin, ameloblastin, and enamelin. In addition, up‐regulation of Fam50a also increased ALP activity and mineralized nodule formation in a dose‐dependent manner. In contrast, knockdown of Fam50a decreased expression levels of enamel matrix protein genes, ALP activity, and mineralized nodule formation. By fluorescence microscopy, endogenous Fam50a protein was found to be localized to the nucleus of ameloblasts. In addition, Fam50a synergistically increased Ambn transactivation by Runx2. Moreover, Fam50a increased binding affinity of Runx2 to Ambn promoter by physically interacting with Runx2. Taken together, these results suggest Fam50a might be a new positive regulator of ameloblast differentiation.
机译：差异化的造釉细胞秘密搪瓷矩阵蛋白质如amelogenin ameloblastin,enamelin。受各种因素的影响。造釉细胞分化调节因子,我们进行了2 d检测页面分析使用鼠标造釉细胞谱系细胞系(malc)培养与成矿介质。有序列相似性50个成员的家庭(Fam50a)期间明显增加malc分化。分泌的造釉细胞中高度表达老鼠牙细菌。表达式的Fam50a监管的表达式釉质基质蛋白基因等amelogenin、ameloblastin enamelin。之外,还是监管Fam50a也增加高山活动和矿化结节形成一个剂量依赖的方式。搪瓷的Fam50a表达水平下降基质蛋白基因,高山活动,矿化结节形成。显微镜,内源性Fam50a蛋白被发现本地化的造釉细胞的细胞核。此外,Ambn Fam50a表现为协同作用增加transactivation Runx2。Ambn Runx2的亲和力增加子的身体与Runx2交互。综上所述,这些研究结果表明Fam50a造釉细胞可能是一个新的积极的监管机构分化。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号