...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Bone marrow adipocytes support hematopoietic stem cell survival
【24h】

Bone marrow adipocytes support hematopoietic stem cell survival

机译：骨髓脂肪细胞支持造血干细胞细胞生存

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In bone marrow (BM), hematopoietic elements are mingled with adipocytes (BM‐A), which are the most abundant stromal component in the niche. BM‐A progressively increase with aging, eventually occupying up to 50% of BM cavities. In this work, the role played by BM‐A was explored by studying primary human BM‐A isolated from hip surgery patients at the molecular level, through microarray analysis, and at the functional level, by assessing their relationship with primary human hematopoietic stem cells (HSC) by the long‐term culture initiating cell (LTC‐IC) assay. Findings demonstrated that BM‐A are capable of supporting HSC survival in the LTC‐IC assay, since after 5 weeks of co‐culture, HSC were still able to proliferate and differentiate. Furthermore, critical molecules such as C‐X‐C motif chemokine 12 (CXCL12), interleukin (IL)‐8, colony‐stimulating factor 3 (CSF3), and leukaemia inhibitory factor (LIF), were expressed at similar levels in BM‐A and in primary human BM mesenchymal stromal cells (BM‐MSC), whereas IL‐3 was higher in BM‐A. Interestingly, BM‐A displayed a different gene expression profile compared with subcutaneous adipose tissue adipocytes (AT‐A) collected from abdominal surgery patients, especially in terms of regulation of lipid metabolism, stemness genes, and white‐to‐brown differentiation pathways. Accordingly, analysis of the gene pathways involved in hematopoiesis regulation showed that BM‐A are more closely related to BM‐MSC than to AT‐A. The present data suggest that BM‐A play a supporting role in the hematopoietic niche and directly sustain HSC survival.
机译：在骨髓(BM)造血元素地理与脂肪细胞(BM),这是最丰富的基质成分的利基。BM逐步增加与老化,最终占据高达50%的BM蛀牙。这项工作,探讨了BM检测所扮演的角色通过研究主要人类BM孤立从臀部手术病人在分子水平上,通过微阵列分析,在功能层面上,通过评估他们与主的关系人类造血干细胞(HSC)长期文化启动细胞(LTC检测IC)测定。研究结果表明,BM的能力支持HSC生存在LTC高集成电路分析5周后以来公司特色文化、HSC仍能够繁殖和分化。此外,地理等关键分子C X C殖民地量刺激因子3 (CSF3)和白血病抑制因子(生活),被表达类似的水平进入BM和人类BM在初级间充质基质细胞(BM MSC)应承担的,而IL 3在BM还是一个更高。不同的基因表达谱与之相比皮下脂肪组织脂肪细胞(A)应承担的从腹部手术患者,收集特别是在调节脂质新陈代谢,具备干细胞基因,和白色至棕色分化途径。的基因通路参与造血作用调节显示,BM更密切有关BM MSC在量比。表明BM的发挥辅助作用造血利基和直接维持HSC生存。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号