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首页> 外文期刊>Journal of Cellular Physiology >Integrated transcriptomic and metabolomic analysis reveals adaptive changes of hibernating retinas
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Integrated transcriptomic and metabolomic analysis reveals adaptive changes of hibernating retinas

机译：集成的转录组和代谢组学分析揭示了冬眠视网膜的适应性变化

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Hibernation is a seasonally adaptive strategy that allows hibernators to live through extremely cold conditions. Despite the profound reduction of blood flow to the retinas, hibernation causes no lasting retinal injury. Instead, hibernators show an increased tolerance to ischemic insults during the hibernation period. To understand the molecular changes of the retinas in response to hibernation, we applied an integrative transcriptome and metabolome analysis to explore changes in gene expression and metabolites of 13‐lined ground squirrel retinas during hibernation. Metabolomic analysis showed a global decrease of ATP synthesis in hibernating retinas. Decreased glucose and galactose, increased beta‐oxidation of carnitine and decreased storage of some amino acids in hibernating retinas indicated a shift of fuel use from carbohydrates to lipids and alternative usage of amino acids. Transcriptomic analysis revealed that the down‐regulated genes were enriched in DNA‐templated transcription and immune‐related functions, while the up‐regulated genes were enriched in mitochondrial inner membrane and DNA packaging‐related functions. We further showed that a subset of genes underwent active alternative splicing events in response to hibernation. Finally, integrative analysis of the transcriptome and metabolome confirmed the shift of fuel use in the hibernating retina by the regulation of catabolism of amino acids and lipids. Through transcriptomic and metabolomic data, our analysis revealed the altered state of mitochondrial oxidative phosphorylation and the shift of energy source in the hibernating retina, advancing our understanding of the molecular mechanisms employed by hibernators. The data will also serve as a useful resource for the ocular and hibernation research communities.
机译：冬眠是一个季节性的适应性策略允许冬眠的黑熊们度过极冷条件。视网膜血流量,冬眠的原因持久的视网膜损伤。增加公差期间缺血性侮辱冬眠期。视网膜的反应的分子变化冬眠,我们应用一个综合转录组和代谢组分析探索基因表达和代谢物的变化13中排列地松鼠视网膜冬眠。减少冬眠ATP合成的视网膜。减少葡萄糖和半乳糖,增加β氧化碱和降低存储一些氨基酸在冬眠的视网膜表示一个转变的燃料的使用碳水化合物脂类和氨基酸的替代用法。转录组分析显示下来量调节基因富集DNA的转录模板化和免疫相关功能,在调节基因富含线粒体内膜和DNA包装的相关功能。基因的一个子集进行了主动可变剪接事件回应冬眠。转录组和代谢组证实了这种转变冬眠的视网膜的燃料使用氨基酸的分解代谢和监管脂质。数据,我们的分析揭示了改变的状态线粒体氧化磷酸化和转变能源的视网膜冬眠,推进我们的理解的分子受雇于冬眠的黑熊们机制。眼也是一个有用的资源和冬眠研究社区。

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