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首页> 外文期刊>Journal of Cellular Physiology >Estrogen receptor β regulates the tumoral suppressor PTEN to modulate pituitary cell growth
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Estrogen receptor β regulates the tumoral suppressor PTEN to modulate pituitary cell growth

机译：雌激素受体βtumoral调节抑制基因PTEN调节垂体细胞生长

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In this study, we focused on ERβ regulation in the adenohypophysis under different estrogenic milieu, by analyzing whether ER modulates the phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression and its subcellular localization on anterior pituitary glands from Wistar rats and GH3 lactosomatotroph cells that over‐expressed ERβ. ERβ was regulated in a cyclic manner, and underwent dynamic changes throughout the estrous cycle, with decreased ERβ+ cells in estrus and under E2 treatment, but increased in ovariectomized rats. In addition, the ERα/β ratio increased in estrus and under E2 stimulation, but decreased in ovariectomized rats. Double immunofluorescence revealed that lactotroph and somatotroph ERβ+ were significantly decreased in estrus. Also, variations in the PTEN expression was observed, which was diminished with high E2 conditions but augmented with low E2 milieu. The subcellular localization of this phosphatase was cell cycle‐dependent, with remarkable changes in the immunostaining pattern: nuclear in arrested pituitary cells but cytoplasmic in stimulated cells, and responding differently to ER agonists, with only DPN being able to increase PTEN expression and retaining it in the nucleus. Finally, ERβ over‐expression increased PTEN with a noticeable subcellular redistribution, and with a significant nuclear signal increase in correlation with an increase of cells in G0/G1 phase. These results showed that E2 is able to inhibit ERβ expression and suggests that the tumoral suppressor PTEN might be one of the signaling proteins by which E2, through ERβ, acts to modulate pituitary cell proliferation, thereby adapting endocrine populations in relation with hormonal necessities.
机译：在这项研究中,我们关注ERβ监管的腺垂体不同雌激素环境,通过分析是否ER调节磷酸酶和tensin同族体删除10号染色体(PTEN)及其表达式亚细胞定位于垂体前叶从Wistar鼠腺体,GH3 lactosomatotroph细胞在表达ERβ。以循环的方式,进行了动态变化整个发情周期,降低ERβ+细胞在发情期和E2治疗,但是增加切除卵巢的老鼠。ERα/β在发情期和E2比例增加刺激,但在切除卵巢的减少老鼠。lactotroph和somatotroph ERβ+在发情明显减少。观察PTEN表达的差异,但是这是减少高E2条件增强E2较低的环境。本地化的磷酸酶是细胞循环量相关,显著的变化疣状模式:核在逮捕但细胞质在刺激垂体细胞细胞,对ER受体激动剂的不同回应,只有DPN能够增加PTEN在细胞核中表达和留住它。最后,ER表达增加PTEN与β一个明显的亚细胞的再分配,一个重要的核信号增强相关性增加细胞G0 / G1阶段。抑制β表达和暗示tumoral抑制PTEN可能之一信号E2蛋白,通过β,行为调节垂体细胞增殖,从而调整内分泌人口的关系荷尔蒙的必需品。

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