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首页> 外文期刊>Journal of Cellular Physiology >Environmental temperature affects physiology and survival of nanosecond pulsed electric field‐treated cells
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Environmental temperature affects physiology and survival of nanosecond pulsed electric field‐treated cells

机译：环境温度影响生理和纳秒脉冲电场的生存场量处理细胞

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摘要

Nanosecond pulsed electric field (nsPEF) is a novel non‐thermal tumor ablation technique. However, how nsPEF affect cell physiology at different environmental temperature is still kept unknown. But this issue is of critical clinical practice relevance. This work aim to investigate how nsPEF treated cancer cells react to different environmental temperatures (0, 4, 25, and 37°C). Their cell viability, apoptosis, mitochondrial membrane potential, and reactive oxygen species (ROS) were examined. Lower temperature resulted in higher apoptosis rate, decreased mitochondria membrane potential, and increased ROS levels. Sucrose and N‐acetylcysteine (NAC) pre‐incubation inhibit ROS generation and increase cell survival, protecting nsPEF‐treated cells from low temperature‐caused cell death. This work provides an experimental basis for hypothermia and fluid transfusion during nsPEF ablation with anesthesia.
机译：纳秒脉冲电场(nsPEF)小说非肿瘤热消融技术。然而,nsPEF如何影响细胞生理学不同的环境温度仍保持未知的。实践的相关性。nsPEF治疗癌细胞如何应对不同环境温度(0、4、25和37°C)。细胞活力、细胞凋亡的线粒体膜电位和活性氧(ROS)检查。在更高的细胞凋亡率,降低线粒体膜电位,增加活性氧的水平。蔗糖和N乙酰半胱氨酸(NAC)提前孵化抑制ROS的生成和增加细胞生存,保护nsPEF治疗细胞低温度引起的细胞死亡。体温过低和流体的实验基础输血期间nsPEF消融麻醉。

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