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首页> 外文期刊>Journal of Cellular Physiology >Osteogenic differentiation potential of mesenchymal stem cells cultured on nanofibrous scaffold improved in the presence of pulsed electromagnetic field
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Osteogenic differentiation potential of mesenchymal stem cells cultured on nanofibrous scaffold improved in the presence of pulsed electromagnetic field

机译：成骨分化的潜力间充质干细胞培养nanofibrous脚手架改善脉冲的存在电磁场

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摘要

Nowadays, tissue engineering by using stem cells in combination with scaffolds and bioactive molecules has made significant contributions to the regeneration of damaged bone tissues. Since the usage of bioactive molecules including, growth factors to induce differentiation is safety limited in clinical applications, and it has also been previously observed that extremely low frequency pulsed electromagnetic fields (PEMF) can be effective in the enhancement of proliferation rate and osteogenic differentiation of stem cells, the aim of this study was investigating the osteoinductive potential of PEMF in combination with Poly(caprolactone) (PCL) nanofibrous scaffold. To achieve this aim, Adipose‐derived mesenchymal stem cells (ADSCs) isolated and characterized and then osteogenic differentiation of them was investigated after culturing on the surface of PCL scaffold under treatments of PEMF, PEMF plus osteogenic medium (OM) and OM. Analysis of common osteogenic markers such as Alizarin red staining, ALP activity, calcium content and four important bone‐related genes in days of 7, 14, and 21 confirmed that the effects of PEMF on the osteogenic differentiation of ADSCs are very similar to the effects of osteogenic medium. Thus, regarding the immunological concerns about the application of bioactive molecules for tissue engineering, PEMF could be a good alternative for osteogenic medium. Although, results were showed a synergetic effect for simultaneous application of PEMF and PCL scaffold in the osteogenesis process of ADSCs. Taking together, ADSCs‐seeded PCL nanofibrous scaffold in combination with PEMF could be a great option for use in bone tissue engineering applications.
机译：现在,通过使用干细胞组织工程结合支架和生物活性分子做出了重要的贡献骨头受损组织的再生。生物活性分子的使用,包括生长因子诱导分化在临床应用安全限制,它之前也被观察到极低频脉冲电磁场(PEMF)可以有效的增强增殖率和成骨分化干细胞,本研究的目的调查的有潜力PEMF结合聚己内酯(PCL)nanofibrous脚手架。脂肪量提取间充质干细胞(ADSCs)孤立和然后成骨的特点分化的研究培养PCL支架表面治疗的PEMF PEMF +成骨的媒介(OM)和“唵”。标记如茜素红染色,高山活动中,钙含量和四个重要地理骨相关基因在7天,14和21确认PEMF上的影响成骨分化ADSCs非常类似于成骨的介质的影响。因此,关于免疫学的担忧生物活性分子组织的应用工程、PEMF可能是一个不错的选择成骨的媒介。同步应用程序的协同作用PEMF和PCL骨支架ADSCs的过程。PCL nanofibrous支架结合PEMF可能是一个伟大的选择用于骨组织工程应用。

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