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首页> 外文期刊>Journal of Cellular Physiology >Aquaporin1 and 3 modification as a result of chondrogenic differentiation of human mesenchymal stem cell
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Aquaporin1 and 3 modification as a result of chondrogenic differentiation of human mesenchymal stem cell

机译：Aquaporin1和3修改的结果chondrogenic人类间叶细胞的分化干细胞

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摘要

Chondrocytes are cells of articular cartilage particularly sensitive to water transport and ionic and osmotic changes from extracellular environment and responsible for the production of the synovial fluid. Aquaporins (AQPs) are a family of water and small solute transport channel proteins identified in several tissues, involved in physiological pathways and in manifold human diseases. In a recent period, AQP1 and 3 seem to have a role in metabolic water regulation in articular cartilage of load bearing joints. The aim of this study was to examine the levels of AQP1 and 3 during the chondrogenic differentiation of human mesenchymal stem cells (MSCs) derived from adipose tissue (AT). For the determination of chondrogenic markers and AQPs levels, glycosaminoglycans (GAGs) quantification, immunocytochemistry, RT‐PCR, and Western blot were used after 0, 7, 14, 21, and 28 days from the start of differentiation. At 21 days, chondrocytes derived from AT‐MSCs were able to produce augmented content of GAGs and significant quantity of SOX‐9, lubricin, aggrecan, and collagen type II, suggesting hyaline cartilage formation, in combination with an increase of AQP3 and AQP1. However, while AQP1 level decreased after 21 days; AQP3 reached higher values at 28 days. The expression of AQP1 and 3 is a manifestation of physiological adaptation of functionally mature chondrocytes able to respond to the change of their internal environment influenced by extracellular matrix. The alteration or loss of expression of AQP1 and 3 could contribute to destruction of chondrocytes and to development of cartilage damage.
机译：软骨细胞是关节软骨的细胞对水运输和特别敏感从细胞外离子和渗透压的变化环境和负责的生产滑液。家庭的水和小溶质运输通道蛋白识别在几个组织,参与的生理途径和多种人类疾病。和3似乎在代谢水作用监管在承重关节软骨关节。在chondrogenic AQP1和3水平人类间充质干细胞的分化(msc)来源于脂肪组织(在)。chondrogenic标记和aqp的决心水平,粘多糖(笑话)量化,免疫细胞化学、地理RT PCR和免疫印迹使用后0、7、14、21、28天的分化的开始。软骨细胞来源于在msc可以产生增强的笑料和重要的内容lubricin袜量量9日,aggrecan,胶原蛋白II型,表明透明软骨的形成,结合增加AQP3和AQP1。减少后21天;值在28天。的生理适应的表现吗功能成熟的软骨细胞能够响应内部环境的变化受细胞外基质的影响。变更或者AQP1的表达和3可能导致软骨细胞的破坏和软骨损伤的发展。

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