A highly efficient method for extracting next‐generation sequencing quality RNA from adipose tissue of recalcitrant animal species

Sharma Davinder; Golla Naresh; Singh DheerOnteru Suneel K.

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A highly efficient method for extracting next‐generation sequencing quality RNA from adipose tissue of recalcitrant animal species

机译：提取的一种高效方法未来一代测序质量RNA顽固的动物的脂肪组织

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The next‐generation sequencing (NGS) based RNA sequencing (RNA‐Seq) and transcriptome profiling offers an opportunity to unveil complex biological processes. Successful RNA‐Seq and transcriptome profiling requires a large amount of high‐quality RNA. However, NGS‐quality RNA isolation is extremely difficult from recalcitrant adipose tissue (AT) with high lipid content and low cell numbers. Further, the amount and biochemical composition of AT lipid varies depending upon the animal species which can pose different degree of resistance to RNA extraction. Currently available approaches may work effectively in one species but can be almost unproductive in another species. Herein, we report a two step protocol for the extraction of NGS quality RNA from AT across a broad range of animal species.

机译：基于第二代测序(上天)应承担的RNA(RNA必经Seq)和转录组测序分析提供了一个机会推出复杂生物过程。转录组分析需要大量‐高品质的RNA。隔离是极其困难的顽固的脂肪组织与高脂质(在)内容和较低的细胞数量。在脂质变化和生化成分根据动物物种构成不同程度的抵制RNA提取。目前可用的方法可以工作但几乎可以有效地在一个物种徒劳的在另一个物种。报告一个两步提取协议门店质量RNA在很大范围内的动物物种。

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来源
《Journal of Cellular Physiology》 |2018年第3期|1971-1974|共4页
作者
Sharma Davinder; Golla Naresh; Singh DheerOnteru Suneel K.;
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作者单位

Molecular Endocrinology, Functional Genomics, and Systems Biology LabICAR‐National Dairy Research;

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原文格式 PDF
正文语种英语
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关键词
Body fat; transcriptomics; RNA isolationGene Expression ProfilingRNA extractionFat contentAdipose TissueAnalyses, Sequenceanimal speciesSequencingRNA;

机译：表达ProfilingRNA extractionFatcontentAdipose TissueAnalyses, SequenceanimalspeciesSequencingRNA;

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A highly efficient method for extracting next‐generation sequencing quality RNA from adipose tissue of recalcitrant animal species

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