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首页> 外文期刊>Journal of Cellular Physiology >CRISPR/Cas9: An RNA‐guided highly precise synthetic tool for plant genome editing
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CRISPR/Cas9: An RNA‐guided highly precise synthetic tool for plant genome editing

机译：CRISPR / Cas9:一个RNA引导高度精确合成植物基因组编辑的工具

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摘要

CRISPR/Cas9 is a newly developed and naturally occurred genome editing tool, which is originally used by bacteria for immune defence. In the past years, it has been quickly employed and modified to precisely edit genome sequences in both plants and animals. Compared with the well‐developed zinc finger nucleases (ZFNs) and transcription activator‐like effector nucleases (TALENs), CRISPR/Cas9 has lots of advantages, including easier to design and implement, higher targeting efficiency, and less expensive. Thus, it is becoming one of the most powerful tools for knockout of an individual gene as well as insertion of one gene and/or control of gene transcription. Studies have shown that CRISPR/Cas9 is a great tool to edit many genes in a variety of plant species, including the model plant species as well as agriculturally important crops, such as cotton, maize, wheat, and rice. CRISPR/Cas9‐based genome editing can be used for plant functional studies and plant improvement to yield, quality, and tolerance to environmental stress.
机译：CRISPR / Cas9自然是新开发的发生基因组编辑工具,这是最初的由细菌用于免疫防御。年,它已经迅速使用和修改准确的编辑在植物基因组序列和动物。锌指核酸酶(ZFNs)和转录核酸酶活化剂‐like执行器(TALENs),CRISPR / Cas9有很多优点,包括易于设计和实现,更高的目标效率,更便宜。成为最强大的工具之一单个基因的敲除等插入一个基因的基因和/或控制转录。CRISPR / Cas9许多基因是一个很好的工具来编辑各种各样的植物物种,包括模型植物物种以及农业重要作物,如棉花、玉米、小麦和大米。CRISPR / Cas9量可用于基于基因组编辑植物功能的研究和改进产量、质量和宽容环境压力。

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