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首页> 外文期刊>Journal of Cellular Physiology >Effect of mono‐(2‐ethylhexyl) phthalate (MEHP) on proliferation of and steroid hormone synthesis in rat ovarian granulosa cells in vitro
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Effect of mono‐(2‐ethylhexyl) phthalate (MEHP) on proliferation of and steroid hormone synthesis in rat ovarian granulosa cells in vitro

机译：mono公/邻苯二甲酸二(2乙基己基)应承担的效果(MEHP)扩散和类固醇激素合成大鼠卵巢颗粒细胞体外

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摘要

This study aimed to examine the proliferation of and secretion by rat ovarian granulosa cells (GCs) treated with mono‐(2‐ethylhexyl) phthalate (MEHP). Ovarian GCs were incubated with MEHP at concentration of 0, 25, 50, 100, and 200?μM for 24?hr. Cell viability was determined using the MTT Cell Proliferation Assay. Progesterone and estradiol production was evaluated by radioimmunoassay (RIA) and the expression of FSHR, PR, and ER was measured by immunocytochemistry. StAR, P450scc, 3β‐HSD, 17β‐HSD, and P 450 arom mRNA levels were determined by RT‐PCR. MEHP markedly attenuated proliferation of GCs, increased expression of sex hormone receptors and key enzymes in progesterone production, and stimulated steroid hormone secretion. The result of these analyses demonstrates that MEHP exposure of GCs may have effects on rat ovarian functions.
机译：本研究旨在检验的扩散并通过大鼠卵巢颗粒细胞分泌(GCs)处理mono的邻苯二甲酸二(2乙基己基)应承担的(MEHP)。浓度为0、25、50、100和200 ?24 ?人力资源。肝癌细胞增殖试验。雌二醇生产评估放射免疫检定法(RIA)的表达FSHR、公关和ER是衡量免疫细胞化学。17βHSD应承担的,450页arom mRNA水平由RT PCR。GCs扩散,增加性的表达激素受体,孕酮的关键酶生产、甾类激素刺激分泌。表明MEHP gc可能的暴露对大鼠卵巢功能的影响。

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