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首页> 外文期刊>Journal of Cellular Physiology >Gap junctional intercellular communication in adipose‐derived stromal/stem cells is cell density‐dependent and positively impacts adipogenic differentiation
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Gap junctional intercellular communication in adipose‐derived stromal/stem cells is cell density‐dependent and positively impacts adipogenic differentiation

机译：差距联接的细胞间通讯脂肪量推导出基质/干细胞细胞密度的依赖和积极的影响脂肪形成的分化

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摘要

Adipose‐derived stromal/stem cells (ASCs) represent a widely used cell source with multi‐lineage differentiation capacity in approaches for tissue engineering and regenerative medicine. Despite the multitude of literature on their differentiation capacity, little is reported about the physiological properties contributing to and controlling the process of lineage differentiation. Direct intercellular communication between adjacent cells via gap junctions has been shown to modulate differentiation processes in other cell types, with connexin 43 (Cx43) being the most abundant isoform of the gap junction‐forming connexins. Thus, in the present study we focused on the expression of Cx43 and gap junctional intercellular communication (GJIC) in human ASCs, and its significance for adipogenic differentiation of these cells. Cx43 expression in ASCs was demonstrated histologically and on the gene and protein expression level, and was shown to be greatly positively influenced by cell seeding density. Functionality of gap junctions was proven by dye transfer analysis in growth medium. Adipogenic differentiation of ASCs was shown to be also distinctly elevated at higher cell seeding densities. Inhibition of GJIC by 18α‐glycyrrhetinic acid (AGA) significantly compromised adipogenic differentiation, as demonstrated by histology, triglyceride quantification, and adipogenic marker gene expression. Flow cytometry analysis showed a lower proportion of cells undergoing adipogenesis when GJIC was inhibited, further indicating the importance of GJIC in the differentiation process. Altogether, this study demonstrates the impact of direct cell–cell communication via gap junctions on the adipogenic differentiation process of ASCs, and may contribute to further integrate direct intercellular crosstalk in rationales for tissue engineering approaches.
机译：脂肪量推导出基质/干细胞(对asc)代表了一种广泛使用的细胞来源多人谱系分化能力为组织工程和方法再生医学。文学的分化能力,关于生理的报道属性和控制做出贡献谱系分化的过程。细胞间相邻之间的通信通过缝隙连接细胞已被证明在其他细胞调节分化过程类型,与联接蛋白43 (Cx43)最多丰富的同种型缝隙连接的形成连接素。交界Cx43的表达和差距在人类对asc细胞间通信(GJIC),对脂肪形成的及其意义这些细胞的分化。在对asc证明组织学检查基因和蛋白表达水平,显示极大的积极影响细胞播种密度。证明了染料转移分析增长媒介。证明是在高也明显升高细胞播种密度。18α甘草次酸(AGA)明显去妥协,通过组织学、甘油三酸酯量化,脂肪形成的标记基因表达式。低比例的细胞发生脂肪形成GJIC抑制时,进一步指示GJIC的差异化的重要性的过程。通过缺口的影响直接的信息交流连接上的脂肪形成的分化进一步对asc的过程,可能导致整合直接细胞间串扰组织工程方法的基本原理。

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