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首页> 外文期刊>Journal of Cellular Physiology >Analysis of extracellular vesicles produced in the biofilm by the dimorphic yeast Pichia fermentans Pichia fermentans
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Analysis of extracellular vesicles produced in the biofilm by the dimorphic yeast Pichia fermentans Pichia fermentans

机译：分析产生的细胞外囊泡生物膜的双晶的毕赤酵母属酵母fermentansPichia fermentans

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摘要

The yeast Pichia fermentans DISAABA 726 strain ( P. fermentans ) is a dimorphic yeast that under different environmental conditions may switch from a yeast‐like to pseudohyphal morphology. We hypothesize that exosomes‐like vesicles (EV) could mediate this rapid modification. EV are membrane‐derived vesicles carrying lipids, proteins, mRNAs and microRNAs and have been recognized as important mediators of intercellular communication. Although it has been assumed for a long time that fungi release EV, knowledge of their functions is still limited. In this work we analyze P. fermentans EV production during growth in two different media containing urea (YCU) or methionine (YCM) where yeast‐like or pseudohyphal morphology are produced. We developed a procedure to extract EV from the neighboring biofilm which is faster and more efficient as compared to the widely used ultracentrifugation method. Differences in morphology and RNA content of EV suggest that they might have an active role during dimorphic transition as response to the growth conditions. Our findings are coherent with a general state of hypoxic stress of the pseudohyphal cells.
机译：p . fermentans)是一个双晶的酵母,在不同的环境条件可能开关从一个酵母pseudohyphal形态。假设液类囊泡(EV)可以调解这种快速修改。膜量推导出囊泡携带脂质,蛋白质,mrna和小分子核糖核酸公认的重要介质细胞间的沟通。假定对于真菌释放EV的很长一段时间,知识的功能仍然是有限的。这项工作我们分析p fermentans电动车生产在两个不同的媒体包含在增长尿素(YCU)或蛋氨酸(YCM)酵母类或pseudohyphal形态。开发了一个程序来提取的电动汽车邻近的生物膜更快和更有效而广泛使用超离心法。形态学和RNA EV建议的内容他们可能在二态的过程中一个活跃的角色转变为应对增长的条件。我们的研究结果是一致的,一般的状态缺氧的压力pseudohyphal细胞。

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