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首页> 外文期刊>Journal of Cellular Physiology >JAK3 and PI3K mediate bovine Interferon‐tau stimulated gene expression in the blood neutrophils
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JAK3 and PI3K mediate bovine Interferon‐tau stimulated gene expression in the blood neutrophils

机译：JAK3和PI3K调解牛干扰素τ刺激血液中的基因表达中性粒细胞

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摘要

Interferon tau, a 23?kDa trophoblast derived protein diffuses out from the uterus into the circulation and leads to the expression of IFNτ stimulated genes viz. ISG15 and OAS1 in blood neutrophils. The IFNτ pathway is species as well as tissue specific. To unsnarl the IFNτ downstream signaling pathway, the blood neutrophils were incubated simultaneously with 10?ng / ml of recombinant bovine interferon tau and the inhibitors of JAK2 (AG490), JAK3 (CP690550), p38 (SB202190), PI3K / Akt (LY294002), and MAPK / Erk (U0126) at specific doses for 4‐hr duration. The IFNτ pathway was determined through real‐time gene expression of ISG15 and OAS1; immunocytochemistry of ISG15; and Western blotting of ISG15, OAS1, pJAK3 and PI3K. The ISG15 and OAS1 expression decreased significantly ( p ??0.001) in the presence of pJAK3 and PI3K inhibitors as compared to a positive control where only interferon tau was used. Immunocytochemistry revealed an attenuated ISG15 response while stimulating blood neutrophils with pJAK3 inhibitor (CP690550) and PI3K inhibitor (LY294002). Similarly, Western blot analysis of neutrophil protein fraction showed weak signals of ISG15, OAS1, pJAK3 and PI3K in the presence of pJAK3 and PI3K inhibitors. The expression profile, immunocytochemistry and western blot analysis revealed a JAK3 and PI3K mediated interferon‐tau stimulated gene expression in blood neutrophils.
机译：干扰素τ,23吗?从子宫到蛋白质扩散出来循环,导致干扰素τ的表达刺激基因即ISG15和OAS1血中性粒细胞。组织特定的。下游信号通路,血液中性粒细胞是同时孵化10 ?和JAK2抑制剂(AG490) JAK3(LY294002)和MAPK / Erk (U0126)在特定的剂量4人力资源持续时间。通过实时的基因表达ISG15 OAS1;西方墨点法ISG15的OAS1, pJAK3和PI3K。ISG15和OAS1表达降低了0.001显著(p & ?)的存在pJAK3和PI3K抑制剂相比积极的控制,只有干扰素τ使用。ISG15响应而刺激血液与pJAK3抑制剂(CP690550)和中性粒细胞PI3K抑制剂(LY294002)。污点分析中性粒细胞蛋白质分数显示ISG15的弱信号,OAS1, pJAK3和PI3K在pJAK3和PI3K的存在抑制剂。免疫细胞化学和免疫印迹分析揭示了JAK3和PI3K干扰素介导的τ刺激血液中性粒细胞的基因表达。

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